ArticleNature chemical biology2010
An in vitro translation, selection and amplification system for peptide nucleic acids.
Article in Nature chemical biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Are we there yet with XNA aptamers?RSC advances · 2026Review
- Omega Nucleic Acids (ΩNA), Ultimate Nucleic Acids for Future Technology.Molecules (Basel, Switzerland) · 2026Review
- Evolution of multivalent supramolecular assemblies of aptamers with target-defined spatial organization.Nature nanotechnology · 2025Article
- Chirality-Promoted Chemical Ligation and Reverse Transcription ofJournal of the American Chemical Society · 2025Article
- Translation of Deoxyribonucleic Acid into Synthetic Alpha Helical Peptides for Darwinian Evolution.JACS Au · 2024Article
- Steering and controlling evolution - from bioengineering to fighting pathogens.Nature reviews. Genetics · 2023Review
- PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs.Analytical chemistry · 2023Article
- Rapid Chemical Ligation of DNA andJournal of the American Chemical Society · 2023Article
- Strategies for developing DNA-encoded libraries beyond binding assays.Nature chemistry · 2022Review
- A mating mechanism to generate diversity for the Darwinian selection of DNA-encoded synthetic molecules.Nature chemistry · 2022Article
- Nonenzymatic polymerase-like template-directed synthesis of acyclic L-threoninol nucleic acid.Nature communications · 2021Article
- Review
- DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials.Topics in current chemistry (Cham) · 2020Review
- A synthetic genetic polymer with an uncharged backbone chemistry based on alkyl phosphonate nucleic acids.Nature chemistry · 2019Article
- "The Smartphone's Guide to the Galaxy": In Situ Analysis in Space.Biosensors · 2018Review
- Evolution of sequence-defined highly functionalized nucleic acid polymers.Nature chemistry · 2018Article
- Aptamers for DNA Damage and Repair.International journal of molecular sciences · 2017Review
- Exploring the Chemistry of Genetic Information Storage and Propagation through Polymerase Engineering.Accounts of chemical research · 2017Review
- Molecular Biodynamers: Dynamic Covalent Analogues of Biopolymers.Accounts of chemical research · 2017Article
- Selection platforms for directed evolution in synthetic biology.Biochemical Society transactions · 2016Review
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4 authors.
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Abstract
Methods to evolve synthetic, rather than biological, polymers could significantly expand the functional potential of polymers that emerge from in vitro evolution. Requirements for synthetic polymer evolution include (i) sequence-specific polymerization of synthetic building blocks on an amplifiable template, (ii) display of the newly translated polymer strand in a manner that allows it to adopt folded structures, (iii) selection of synthetic polymer libraries for desired binding or catalytic properties and (iv) amplification of template sequences that survive selection in a manner that allows subsequent translation. Here we report the development of such a system for peptide nucleic acids (PNAs) using a set of 12 PNA pentamer building blocks. We validated the system by performing six iterated cycles of translation, selection and amplification on a library of 4.3 x 10(8) PNA-encoding DNA templates and observed >1,000,000-fold overall enrichment of a template encoding a biotinylated (streptavidin-binding) PNA. These results collectively provide an experimental foundation for PNA evolution in the laboratory.
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