ArticleNature chemical biology2010
Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont.
Article in Nature chemical biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 141 citations in OpenAlex.
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- Biochemical characterization of the α-1,3-mannosidase AnGH92A from Aspergillus nidulans.Scientific reports · 2026Article
- Probing the Broad-Spectrum Virus-Neutralizing Epitopes Using the High-Density Oligomannose-Conjugates and Carbohydrate Microarrays.Bioconjugate chemistry · 2025Article
- Shaping the Glycan Landscape: Hidden Relationships between Linkage and Ring Distortions Induced by Carbohydrate-Active Enzymes.Journal of the American Chemical Society · 2025Article
- Emerging gut microbial glycoside hydrolase inhibitors.RSC chemical biology · 2025Review
- Genomic and phenotypic characterisation of Leeuwenhoekiella obamensis sp. nov., a novel marine bacterium isolated from the surface water of a Japanese fishing port.Antonie van Leeuwenhoek · 2025Article
- Glycosidic linkages of fungus polysaccharides influence the anti-inflammatory activity in mice.Journal of advanced research · 2025Article
- Polysaccharide utilization loci encoded DUF1735 likely functions as membrane-bound spacer for carbohydrate active enzymes.FEBS open bio · 2024Article
- Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic.ISME communications · 2023Article
- Genomics of soil depth niche partitioning in the Thaumarchaeota family Gagatemarchaeaceae.Nature communications · 2023Article
- Inducible CRISPR-targeted "knockdown" of human gutProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Rice Endophyte-Derived α-Mannosidase ShAM1 Degrades Host Cell Walls To Activate DAMP-Triggered Immunity against Disease.Microbiology spectrum · 2023Article
- Structural and functional characterization of a multi-domain GH92 α-1,2-mannosidase from Neobacillus novalis.Acta crystallographica. Section D, Structural biology · 2023Article
- Glycoprotein In Vitro N-Glycan Processing Using Enzymes Expressed inMolecules (Basel, Switzerland) · 2023Article
- Biochemical characteristics of point mutated Capra hircus lysosome α-mannosidase.The Journal of veterinary medical science · 2023Article
- Design and directed evolution of noncanonical β-stereoselective metalloglycosidases.Nature communications · 2022Article
- PlantProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Glycoside hydrolase from the GH76 family indicates that marine Salegentibacter sp. Hel_I_6 consumes alpha-mannan from fungi.The ISME journal · 2022Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 7 institutions in 4 countries.
Funding
Abstract
Colonic bacteria, exemplified by Bacteroides thetaiotaomicron, play a key role in maintaining human health by harnessing large families of glycoside hydrolases (GHs) to exploit dietary polysaccharides and host glycans as nutrients. Such GH family expansion is exemplified by the 23 family GH92 glycosidases encoded by the B. thetaiotaomicron genome. Here we show that these are alpha-mannosidases that act via a single displacement mechanism to utilize host N-glycans. The three-dimensional structure of two GH92 mannosidases defines a family of two-domain proteins in which the catalytic center is located at the domain interface, providing acid (glutamate) and base (aspartate) assistance to hydrolysis in a Ca(2+)-dependent manner. The three-dimensional structures of the GH92s in complex with inhibitors provide insight into the specificity, mechanism and conformational itinerary of catalysis. Ca(2+) plays a key catalytic role in helping distort the mannoside away from its ground-state (4)C(1) chair conformation toward the transition state.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.