ArticleThe Journal of biological chemistry2010
Myosin X regulates sealing zone patterning in osteoclasts through linkage of podosomes and microtubules.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- Egg yolk hydrolysate shows moderate impact on osteoporosis in ovariectomized rats.Journal of the science of food and agriculture · 2026Article
- Formation of Membrane Domains via Actin Waves: A Fundamental Principle in the Generation of Dynamic Structures in Phagocytes.International journal of molecular sciences · 2025Review
- The role of tunneling nanotubes during early stages of HIV infection and reactivation: implications in HIV cure.NeuroImmune pharmacology and therapeutics · 2023Review
- Super-resolution microscopy reveals nanoscale architecture and regulation of podosome clusters in primary macrophages.iScience · 2022Article
- Proteomics Profiling of Osteoporosis and Osteopenia Patients and Associated Network Analysis.International journal of molecular sciences · 2022Article
- The Effects of Mechanical Stretch on Integrins and Filopodial-Associated Proteins in Normal and Glaucomatous Trabecular Meshwork Cells.Frontiers in cell and developmental biology · 2022Article
- The dynactin subunit DCTN1 controls osteoclastogenesis via the Cdc42/PAK2 pathway.Experimental & molecular medicine · 2020Article
- Phenotypic and Functional Alterations in Tunneling Nanotubes Formed by Glaucomatous Trabecular Meshwork Cells.Investigative ophthalmology & visual science · 2019Article
- Article
- Lack of Myosin X Enhances Osteoclastogenesis and Increases Cell Surface Unc5b in Osteoclast-Lineage Cells.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.Investigative ophthalmology & visual science · 2019Article
- Induction of Krüppel-like factor 2 reduces K/BxN serum-induced arthritis.Journal of cellular and molecular medicine · 2019Article
- Myosins in Osteoclast Formation and Function.Biomolecules · 2018Review
- Myosin X is required for efficient melanoblast migration and melanoma initiation and metastasis.Scientific reports · 2018Article
- The Sealing Zone in Osteoclasts: A Self-Organized Structure on the Bone.International journal of molecular sciences · 2018Review
- Genetic Dissection of Trabecular Bone Structure with Mouse Intersubspecific Consomic Strains.G3 (Bethesda, Md.) · 2017Article
- Regulation of Osteoclast Differentiation by Myosin X.Scientific reports · 2017Article
- Looking for Calcium Phosphate Composite Suitable to Study Osteoclast Endocytosis: Preliminary Observations.Translational medicine @ UniSa · 2016Article
- In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts.Scientific reports · 2016Article
- Cytoplasmic hnRNPK interacts with GSK3β and is essential for the osteoclast differentiation.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Osteoclasts use actin-rich attachment structures in place of focal adhesions for adherence to bone and non-bone substrates. On glass, osteoclasts generate podosomes, foot-like processes containing a core of F-actin and regulatory proteins that undergo high turnover. To facilitate bone resorption, osteoclasts generate an actin-rich sealing zone composed of densely packed podosome-like units. Patterning of both podosomes and sealing zones is dependent upon an intact microtubule system. A role for unconventional myosin X (Myo10), which can bind actin, microtubules, and integrins, was examined in osteoclasts. Immunolocalization showed Myo10 to be associated with the outer edges of immature podosome rings and sealing zones, suggesting a possible role in podosome and sealing zone positioning. Further, complexes containing both Myo10 and beta-tubulin were readily precipitated from osteoclasts lysates. RNAi-mediated suppression of Myo10 led to decreased cell and sealing zone perimeter, along with decreased motility and resorptive capacity. Further, siRNA-treated cells could not properly position podosomes following microtubule disruption. Osteoclasts overexpressing dominant negative Myo10 microtubule binding domains (MyTH4) showed a similar phenotype. Conversely, overexpression of full-length Myo10 led to increased formation of podosome belts along with larger sealing zones and enhanced bone resorptive capacity. These studies suggest that Myo10 plays a role in osteoclast attachment and podosome positioning by direct linkage of actin to the microtubule network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.