ArticleNucleic acids research2010
DNA chirality-dependent stimulation of topoisomerase IV activity by the C-terminal AAA+ domain of FtsK.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Mechanism of negative supercoil relaxation by Topoisomerase IV.Nucleic acids research · 2026Article
- Temporospatial control of topoisomerases by essential cellular processes.Current opinion in microbiology · 2024Review
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Single-nucleotide resolution detection of Topo IV cleavage activity in theFrontiers in microbiology · 2023Article
- SepF supports the recruitment of the DNA translocase SftA to the Z-ring.Molecular microbiology · 2022Article
- Extended sister-chromosome catenation leads to massive reorganization of the E. coli genome.Nucleic acids research · 2022Article
- Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates.Biology · 2021Article
- Cohesion of Sister Chromosome Termini during the Early Stages of Sporulation in Bacillus subtilis.Journal of bacteriology · 2020Article
- Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP.Nature communications · 2020Article
- Division-induced DNA double strand breaks in the chromosome terminus region of Escherichia coli lacking RecBCD DNA repair enzyme.PLoS genetics · 2017Article
- Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.PLoS genetics · 2016Article
- A 1 MDa protein complex containing critical components of the Escherichia coli divisome.Scientific reports · 2015Article
- A cell cycle-controlled redox switch regulates the topoisomerase IV activity.Genes & development · 2015Article
- Differential management of the replication terminus regions of the two Vibrio cholerae chromosomes during cell division.PLoS genetics · 2014Article
- The MukB-ParC interaction affects the intramolecular, not intermolecular, activities of topoisomerase IV.The Journal of biological chemistry · 2013Article
- Molecular motors for DNA translocation in prokaryotes.Current opinion in biotechnology · 2012Review
- All tangled up: how cells direct, manage and exploit topoisomerase function.Nature reviews. Molecular cell biology · 2011Review
- A septal chromosome segregator protein evolved into a conjugative DNA-translocator protein.Mobile genetic elements · 2011Article
- A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.PloS one · 2011Article
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Authors and funding
2 authors.
Funding
Abstract
We have studied the stimulation of topoisomerase IV (Topo IV) by the C-terminal AAA+ domain of FtsK. These two proteins combine to assure proper chromosome segregation in the cell. Stimulation of Topo IV activity was dependent on the chirality of the DNA substrate: FtsK stimulated decatenation of catenated DNA and relaxation of positively supercoiled [(+)ve sc] DNA, but inhibited relaxation of negatively supercoiled [(-)ve sc] DNA. The DNA translocation activity of FtsK was not required for stimulation, but was required for inhibition. DNA chirality did not affect any of the activities of FtsK, suggesting that FtsK possesses an inherent Topo IV stimulatory activity that is presumably mediated by protein-protein interactions, the stability of Topo IV on the DNA substrate dictated the effect observed. Inhibition occurs because FtsK can strip distributively acting topoisomerase off (-)ve scDNA, but not from either (+)ve scDNA or catenated DNA where the enzyme acts processively. Our analyses suggest that FtsK increases the efficiency of trapping of the transfer segment of DNA during the catalytic cycle of the topoisomerase.
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