ArticleNucleic acids research2010
Mapping of interaction sites of the Schizosaccharomyces pombe protein Translin with nucleic acids and proteins: a combined molecular genetics and bioinformatics study.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Translin facilitates RNA polymerase II dissociation and suppresses genome instability during RNase H2- and Dicer-deficiency.PLoS genetics · 2022Article
- Translin: A multifunctional protein involved in nucleic acid metabolism.Journal of biosciences · 2019Review
- Translin and Trax differentially regulate telomere-associated transcript homeostasis.Oncotarget · 2016Article
- Characterization of a plant (rice) translin and its comparative analysis with human translin.Planta · 2014Article
- Conformational transitions in human translin enable nucleic acid binding.Nucleic acids research · 2013Article
- Structural basis for duplex RNA recognition and cleavage by Archaeoglobus fulgidus C3PO.Nature structural & molecular biology · 2013Article
- Molecular evolution of translin superfamily proteins within the genomes of eubacteria, archaea and eukaryotes.Journal of molecular evolution · 2012Article
- Computational prediction of protein-protein complexes.BMC research notes · 2012Article
- Low-resolution structure of Drosophila translin.FEBS open bio · 2012Article
- Identification of nucleic acid binding sites on translin-associated factor X (TRAX) protein.PloS one · 2012Article
- Multimeric assembly and biochemical characterization of the Trax-translin endonuclease complex.Nature structural & molecular biology · 2011Article
- Search for limiting factors in the RNAi pathway in silkmoth tissues and the Bm5 cell line: the RNA-binding proteins R2D2 and Translin.PloS one · 2011Article
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Authors and funding
6 authors.
Funding
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Abstract
Translin is a single-stranded RNA- and DNA-binding protein, which has been highly conserved in eukaryotes, from man to Schizosaccharomyces pombe. TRAX is a Translin paralog associated with Translin, which has coevolved with it. We generated structural models of the S. pombe Translin (spTranslin), based on the solved 3D structure of the human ortholog. Using several bioinformatics computation tools, we identified in the equatorial part of the protein a putative nucleic acids interaction surface, which includes many polar and positively charged residues, mostly arginines, surrounding a shallow cavity. Experimental verification of the bioinformatics predictions was obtained by assays of nucleic acids binding to amino acid substitution variants made in this region. Bioinformatics combined with yeast two-hybrid assays and proteomic analyses of deletion variants, also identified at the top of the spTranslin structure a region required for interaction with spTRAX, and for spTranslin dimerization. In addition, bioinformatics predicted the presence of a second protein-protein interaction site at the bottom of the spTranslin structure. Similar nucleic acid and protein interaction sites were also predicted for the human Translin. Thus, our results appear to generally apply to the Translin family of proteins, and are expected to contribute to a further elucidation of their functions.
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