ArticleApplied and environmental microbiology2010
Comparison of two optical-density-based methods and a plate count method for estimation of growth parameters of Bacillus cereus.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 110 citations in OpenAlex.
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- Study of grapevine endophytes' interaction with the host and their potential as biocontrol agents as sustainable alternative to agrochemicals.Frontiers in microbiology · 2026Article
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- gcplyr: an R package for microbial growth curve data analysis.BMC bioinformatics · 2024Article
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- Absence of Ribosome Modulation Factor Alters Growth and Competitive Fitness of Escherichia coli.Microbiology spectrum · 2022Article
- Mass spectrometry-based identification of bacteria isolated from industrially contaminated site in Salamanca (Mexico) and evaluation of their potential for DDT degradation.Folia microbiologica · 2021Article
- Antimicrobial properties, compressive strength and fluoride release capacity of essential oil-modified glass ionomer cements-an in vitro study.Clinical oral investigations · 2021Article
- Biological Inoculant of Salt-Tolerant Bacteria for Plant Growth Stimulation under Different Saline Soil Conditions.Journal of microbiology and biotechnology · 2021Article
- Bacterial-like nitric oxide synthase in the haloalkaliphilic archaeon Natronomonas pharaonis.MicrobiologyOpen · 2020Article
- Mucosal-Associated Invariant T Cells Develop an Innate-Like Transcriptomic Program in Anti-mycobacterial Responses.Frontiers in immunology · 2020Article
- A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions.Scientific reports · 2019Article
- Effect of microbial sanitizers for reducing biofilm formation ofFood science and biotechnology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative microbiological models predicting proliferation of microorganisms relevant for food safety and/or food stability are useful tools to limit the need for generation of biological data through challenge testing and shelf-life testing. The use of these models requires quick and reliable methods for the generation of growth data and estimation of growth parameters. Growth parameter estimation can be achieved using methods based on plate counting and methods based on measuring the optical density. This research compares the plate count method with two optical density methods, namely, the 2-fold dilution (2FD) method and the relative rate to detection (RRD) method. For model organism Bacillus cereus F4810/72, the plate count method and both optical density methods gave comparable estimates for key growth parameters. Values for the maximum specific growth rate (mu(max)) derived by the 2FD method and by the RRD method were of the same order of magnitude, but some marked differences between the two approaches were apparent. Whereas the 2FD method allowed the derivation of values for lag time (lambda) from the data, this was not possible with the RRD method. However, the RRD method gave many more data points per experiment and also gave more data points close to the growth boundary. This research shows that all three proposed methods can be used for parameter estimation but that the choice of method depends on the objectives of the research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.