ArticleProceedings of the National Academy of Sciences of the United States of America2009
Small RNA as global regulator of carbon catabolite repression in Pseudomonas aeruginosa.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers, 2 of them syntheses that pooled it.
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Who cites it
140 citing papers in PubMed, 2 syntheses or guidelines pooled it, 263 citations in OpenAlex.
- Implications of carbon catabolite repression for plant-microbe interactions.Plant communications · 2022Pooled it
- Carbohydrates Metabolic Signatures in Immune Cells: Response to Infection.Frontiers in immunology · 2022Pooled it
- Structure and conformational dynamics of the Pseudomonas CbrA transceptor.Protein science : a publication of the Protein Society · 2026Article
- Structure and conformational dynamics of thebioRxiv : the preprint server for biology · 2026Article
- Biosurfactant-Producing Bacteria Isolated from a Microbial Consortium Previously Subjected to Adaptive Laboratory Evolution in Oily Sludge.Microorganisms · 2026Article
- GlnK Regulates the Type III Secretion System by Modulating NtrB-NtrC Homeostasis inMicroorganisms · 2026Article
- From environmental signals to adaptive phenotypes: signal-responsive regulation and network logic of bacterial small RNAs.FEMS microbiology reviews · 2026Review
- Carbon source-driven downregulation of the sRNA AmiL enhancesVirulence · 2025Article
- Carbon source utilization regulates biofilm formation and plant-beneficial interactions ofiScience · 2025Article
- The Catabolite Repression Control Protein Crc Regulates the Type III Secretion System Through the Adenylate Cyclase CyaB inMicroorganisms · 2025Article
- The link of carbon catabolite repression elements, small RNAs CrcY and CrcZ and polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440.Biotechnology for biofuels and bioproducts · 2025Article
- All-at-once RNA folding with 3D motif prediction framed by evolutionary information.Nature methods · 2025Article
- Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters.Science advances · 2025Article
- Loss of LasR function leads to decreased repression ofJournal of bacteriology · 2025Article
- Combinatorial control ofmSystems · 2024Article
- Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic compounds.Microbial biotechnology · 2024Article
- Sphingosine induction of theJournal of bacteriology · 2024Article
- Aminoglycoside uptake, stress, and potentiation in Gram-negative bacteria: new therapies with old molecules.Microbiology and molecular biology reviews : MMBR · 2023Review
- Fructose promotes pyoluteorin biosynthesis via the CbrAB-CrcZ-Hfq/Crc pathway in the biocontrol strainSynthetic and systems biotechnology · 2023Article
- Simultaneous carbon catabolite repression governs sugar and aromatic co-utilization inApplied and environmental microbiology · 2023Article
80 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the metabolically versatile bacterium Pseudomonas aeruginosa, the RNA-binding protein Crc is involved in catabolite repression of a range of degradative genes, such as amiE (encoding aliphatic amidase). We found that a CA-rich sequence (termed CA motif) in the amiE translation initiation region was important for Crc binding. The small RNA CrcZ (407 nt) containing 5 CA motifs was able to bind the Crc protein with high affinity and to remove it from amiE mRNA in vitro. Overexpression of crcZ relieved catabolite repression in vivo, whereas a crcZ mutation pleiotropically prevented the utilization of several carbon sources. The sigma factor RpoN and the CbrA/CbrB two-component system, which is known to maintain a healthy carbon-nitrogen balance, were necessary for crcZ expression. During growth on succinate, a preferred carbon source, CrcZ expression was low, resulting in catabolite repression of amiE and other genes under Crc control. By contrast, during growth on mannitol, a poor carbon source, elevated CrcZ levels correlated with relief of catabolite repression. During growth on glucose, an intermediate carbon source, CrcZ levels and amiE expression were intermediate between those observed in succinate and mannitol media. Thus, the CbrA-CbrB-CrcZ-Crc system allows the bacterium to adapt differentially to various carbon sources. This cascade also regulated the expression of the xylS (benR) gene, which encodes a transcriptional regulator involved in benzoate degradation, in an analogous way, confirming this cascade's global role.
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