ArticleProceedings of the National Academy of Sciences of the United States of America2010
Plant homologs of the Plasmodium falciparum chloroquine-resistance transporter, PfCRT, are required for glutathione homeostasis and stress responses.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
78 citing papers in PubMed, 180 citations in OpenAlex.
- Development and validation of HILIC-MS/MS method for the analysis of glutathione metabolism in cell cultures.Analytical and bioanalytical chemistry · 2026Article
- Article
- Glutathione accelerates the cell cycle and cellular reprogramming in plant regeneration.Developmental cell · 2025Article
- Adaptations and metabolic evolution of myzozoan protists across diverse lifestyles and environments.Microbiology and molecular biology reviews : MMBR · 2024Review
- Untying the knot: Unraveling genetic mechanisms behind black knot disease resistance inPlant-environment interactions (Hoboken, N.J.) · 2024Article
- Glutathione accelerates the cell cycle and cellular reprogramming in plant regeneration.bioRxiv : the preprint server for biology · 2024Article
- Development of KASP markers assisted with soybean drought tolerance in the germination stage based on GWAS.Frontiers in plant science · 2024Article
- The Main Functions of Plastids.Methods in molecular biology (Clifton, N.J.) · 2024Review
- The Toxoplasma plant-like vacuolar compartment (PLVAC).The Journal of eukaryotic microbiology · 2022Review
- Organelle-specific localization of glutathione in plants grown under different light intensities and spectra.Histochemistry and cell biology · 2022Review
- Transcript profiling of glutathione metabolizing genes reveals abiotic stress and glutathione-specific alteration inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress.International journal of molecular sciences · 2022Review
- Transcriptional profiling reveals conserved and species-specific plant defense responses during the interaction of Physcomitrium patens with Botrytis cinerea.Plant molecular biology · 2021Article
- Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification.Metabolites · 2021Review
- Live monitoring of plant redox and energy physiology with genetically encoded biosensors.Plant physiology · 2021Review
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Subcellular Roles of Glutathione in Mediating Plant Defense during Biotic Stress.Plants (Basel, Switzerland) · 2020Review
- Chloroplast-associated molecular patterns as concept for fine-tuned operational retrograde signalling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Structural and evolutionary analyses of the Plasmodium falciparum chloroquine resistance transporter.Scientific reports · 2020Article
- Conserved defense responses between maize and sorghum to Exserohilum turcicum.BMC plant biology · 2020Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
20 authors at 6 institutions in 3 countries.
Funding
Abstract
In Arabidopsis thaliana, biosynthesis of the essential thiol antioxidant, glutathione (GSH), is plastid-regulated, but many GSH functions, including heavy metal detoxification and plant defense activation, depend on cytosolic GSH. This finding suggests that plastid and cytosol thiol pools are closely integrated and we show that in Arabidopsis this integration requires a family of three plastid thiol transporters homologous to the Plasmodium falciparum chloroquine-resistance transporter, PfCRT. Arabidopsis mutants lacking these transporters are heavy metal-sensitive, GSH-deficient, and hypersensitive to Phytophthora infection, confirming a direct requirement for correct GSH homeostasis in defense responses. Compartment-specific measurements of the glutathione redox potential using redox-sensitive GFP showed that knockout of the entire transporter family resulted in a more oxidized glutathione redox potential in the cytosol, but not in the plastids, indicating the GSH-deficient phenotype is restricted to the cytosolic compartment. Expression of the transporters in Xenopus oocytes confirmed that each can mediate GSH uptake. We conclude that these transporters play a significant role in regulating GSH levels and the redox potential of the cytosol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.