ArticleProceedings of the National Academy of Sciences of the United States of America2010
Measurement of mass, density, and volume during the cell cycle of yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 286 citations in OpenAlex.
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- Cell division timing shapes the morphology and size of nascent multicellular organisms.bioRxiv : the preprint server for biology · 2026Article
- Single-cell mass-density measurements using microchannel gradient centrifugation.Scientific reports · 2026Article
- Phenotypic heterogeneity in a batch culture of Chlamydomonas reinhardtii with different light tolerances.PloS one · 2026Article
- Complex Kinetic Models Predict β-Carotene Production and Reveal Flux Limitations in RecombinantACS synthetic biology · 2025Article
- Brachiopods and forams reduced calcification costs through morphological simplification during mass extinction events.Nature ecology & evolution · 2025Article
- A Miniaturized Percoll Gradient Method for Isolation of Quiescent Cells of Yeast.Bio-protocol · 2025Article
- High-Throughput Measurement of Single-Fission Yeast Cell Volume Using Fluorescence Exclusion.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.Theory in biosciences = Theorie in den Biowissenschaften · 2024Article
- Development of chemically defined media for3 Biotech · 2023Article
- Dry mass photometry of single bacteria using quantitative wavefront microscopy.Biophysical journal · 2023Article
- When Phased without Water: Biophysics of Cellular Desiccation, from Biomolecules to Condensates.Chemical reviews · 2023Review
- MorphoSim: an efficient and scalable phase-field framework for accurately simulating multicellular morphologies.NPJ systems biology and applications · 2023Article
- Screening ofFrontiers in microbiology · 2023Article
- TORC1 and PKA activity towards ribosome biogenesis oscillates in synchrony with the budding yeast cell cycle.Journal of cell science · 2022Article
- Extending applications of AFM to fluidic AFM in single living cell studies.Journal of cellular physiology · 2022Review
- In vitro cell cycle oscillations exhibit a robust and hysteretic response to changes in cytoplasmic density.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The correlation between cell and nucleus size is explained by an eukaryotic cell growth model.PLoS computational biology · 2022Article
- Microfluidic Single-Cell Analytics.Advances in biochemical engineering/biotechnology · 2022Article
42 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cell growth comprises changes in both mass and volume--two processes that are distinct, yet coordinated through the cell cycle. Understanding this relationship requires a means for measuring each of the cell's three basic physical parameters: mass, volume, and the ratio of the two, density. The suspended microchannel resonator weighs single cells with a precision in mass of 0.1% for yeast. Here we use the suspended microchannel resonator with a Coulter counter to measure the mass, volume, and density of budding yeast cells through the cell cycle. We observe that cell density increases prior to bud formation at the G1/S transition, which is consistent with previous measurements using density gradient centrifugation. To investigate the origin of this density increase, we monitor relative density changes of growing yeast cells. We find that the density increase requires energy, function of the protein synthesis regulator target of rapamycin, passage through START (commitment to cell division), and an intact actin cytoskeleton. Although we focus on basic cell cycle questions in yeast, our techniques are suitable for most nonadherent cells and subcellular particles to characterize cell growth in a variety of applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.