ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2010
Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-duration of symptoms significantly affects detection rate.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 2 of them syntheses that pooled it.
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Who cites it
39 citing papers in PubMed, 2 syntheses or guidelines pooled it, 69 citations in OpenAlex.
- Systematic review on the association between respiratory virus real-time PCR cycle threshold values and clinical presentation or outcomes.The Journal of antimicrobial chemotherapy · 2021Pooled it
- The Etiological Role of Common Respiratory Viruses in Acute Respiratory Infections in Older Adults: A Systematic Review and Meta-analysis.The Journal of infectious diseases · 2020Pooled it
- Randomised controlled trial of rhinothermy for treatment of the common cold: a feasibility study.BMJ open · 2018Trial
- Randomized, double-blind, placebo-controlled clinical trial to assess the safety and effectiveness of a novel dual-action oral topical formulation against upper respiratory infections.BMC infectious diseases · 2017Trial
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
- Article
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- Incidence of Respiratory Syncytial Virus Infection in Older Adults: Limitations of Current Data.Infectious diseases and therapy · 2023Review
- Development of a diagnostic assay by three-tube multiplex real-time PCR for simultaneous detection of nine microorganisms causing acute respiratory infections.Scientific reports · 2022Article
- Prevention of SARS-CoV-2 (COVID-19) transmission in residential aged care using ultraviolet light (PETRA): a two-arm crossover randomised controlled trial protocol.BMC infectious diseases · 2021Article
- Respiratory microbes detected in hospitalized adults with acute respiratory infections: associations between influenza A(H1N1)pdm09 virus and intensive care unit admission or fatal outcome in Vietnam (2015-2017).BMC infectious diseases · 2021Article
- Advances in laboratory assays for detecting human metapneumovirus.Annals of translational medicine · 2020Review
- Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China.Biomedical and environmental sciences : BES · 2019Article
- Microbiological findings in bronchoalveolar lavage fluid from lung transplant patients in Sweden.Transplant infectious disease : an official journal of the Transplantation Society · 2018Article
- Rapid detection of respiratory organisms with the FilmArray respiratory panel in a large children's hospital in China.BMC infectious diseases · 2018Article
- Do asymptomatic respiratory viral infections occur?Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Article
- Respiratory viral infections are underdiagnosed in patients with suspected sepsis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Respiratory Multiplex Polymerase Chain Reaction: An Important Diagnostic Tool in Immunocompromised Patients.Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine · 2017Article
- Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples.Molecular and cellular probes · 2015Article
- Surveillance of acute respiratory infections among outpatients: A pilot study in Isfahan city.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNucleic acid amplification techniques have improved the diagnostic possibilities in respiratory tract infections, although their clinical applicability is not yet fully defined. We have evaluated a multiplex real-time PCR method for the detection of 13 respiratory viruses and 2 bacteria (Mycoplasma and Chlamydophila) in a clinical setting.
objectivesThe aim of the present study was to evaluate the diagnostic performance and clinical use of a novel multiplex PCR method in adults with community-acquired respiratory viral infection, and the impact of duration of symptoms on detection rates. STUDY
designNasopharyngeal swab samples were prospectively collected from 209 adult outpatients with respiratory infections and 100 asymptomatic controls.
resultsAn infectious agent was identified in 43% of samples from patients and 2% of asymptomatic controls. The detection rate was significantly higher in samples from patients with a duration of symptoms of 6 days or less (51%) than in samples from patients with a duration of symptoms of 7 days or more (30%, p<0.01). For human corona viruses, and influenza virus A and B there was a correlation between the amount of virus in each patient sample as measured Ct values and duration of symptoms.
conclusionsDuration of symptoms significantly affects the detection rate of respiratory pathogens by multiplex real-time PCR in nasopharyngeal swab samples from adult patients with respiratory infections. Our finding should be taken into account when using these tests in clinical practise.
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