ArticleReproductive biology and endocrinology : RB&E2010
Identification of calcium-binding proteins associated with the human sperm plasma membrane.
Article in Reproductive biology and endocrinology : RB&E, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- Calcium Channels and Modulators as Potential Therapeutic Targets for Contraceptives and Male Fertility: A Scoping Review.Open access journal of contraception · 2025Review
- Review
- Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution.International journal of molecular sciences · 2021Article
- The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage.Proceedings. Biological sciences · 2016Article
- Amblyomma americanum tick calreticulin binds C1q but does not inhibit activation of the classical complement cascade.Ticks and tick-borne diseases · 2015Article
- Expression of α-subunit of α-glucosidase II in adult mouse brain regions and selected organs.Journal of neuroscience research · 2015Article
- Endoplasmic reticulum chaperones and their roles in the immunogenicity of cancer vaccines.Frontiers in oncology · 2014Review
- Human sperm tail proteome suggests new endogenous metabolic pathways.Molecular & cellular proteomics : MCP · 2013Article
- Calciomics: integrative studies of Ca2+-binding proteins and their interactomes in biological systems.Metallomics : integrated biometal science · 2013Review
- Serum amyloid P component: a new biomarker for low sperm concentration?Asian journal of andrologyArticle
- Proteomic analysis reveals dysregulated cell signaling in ejaculated spermatozoa from infertile men.Asian journal of andrologyArticle
- Characterization of MAGEG2 with testis-specific expression in mice.Asian journal of andrologyArticle
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe precise composition of the human sperm plasma membrane, the molecular interactions that define domain specific functions, and the regulation of membrane associated proteins during the capacitation process, still remain to be fully understood. Here, we investigated the repertoire of calcium-regulated proteins associated with the human sperm plasma membrane.
methodsSurface specific radioiodination was combined with two-dimensional gel electrophoresis, a 45Ca-overlay assay, computer assisted image analysis and mass spectrometry to identify calcium-binding proteins exposed on the human sperm surface.
resultsNine acidic 45Ca-binding sperm proteins were excised from stained preparative 2D gels and identified by mass spectrometry. Five of the calcium binding proteins; HSPA2 (HSP70-1), HSPA5 (Bip), HYOU1 (ORP150), serum amyloid P-component (SAP) and protein kinase C substrate 80K-H (80K-H) were found to be accessible to Iodo-Bead catalyzed 125I-labelling on the surface of intact human sperm. Agglutination and immunofluorescence analysis confirmed that SAP is situated on the plasma membrane of intact, motile sperm as well as permeabilized cells. Western blot analysis showed increased phosphorylation of human sperm 80K-H protein following in vitro capacitation. This is the first demonstration of the 80K-H protein in a mammalian sperm.
conclusionThe presence of SAP on the surface of mature sperm implies that SAP has a physiological role in reproduction, which is thought to be in the removal of spermatozoa from the female genital tract via phagocytosis. Since 80K-H is a Ca2+-sensor recently implicated in the regulation of both inositol 1,4,5-trisphosphate receptor and transient receptor potential (TRP) cation channel activities, its detection in sperm represents the first direct signaling link between PKC and store-operated calcium channels identified in human sperm.
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