ArticleMolecular plant pathology2010
Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime.
Article in Molecular plant pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 53 citations in OpenAlex.
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions.Frontiers in plant science · 2023Article
- Epigenetic Changes and Transcriptional Reprogramming Upon Woody Plant Grafting for Crop Sustainability in a Changing Environment.Frontiers in plant science · 2020Review
- Citrus Genetic Engineering for Disease Resistance: Past, Present and Future.International journal of molecular sciences · 2019Review
- Review
- Accumulation of 24 nucleotide transgene-derived siRNAs is associated with crinivirus immunity in transgenic plants.Molecular plant pathology · 2018Article
- Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.Transgenic research · 2018Article
- Review
- Biosafety considerations of RNAi-mediated virus resistance in fruit-tree cultivars and in rootstock.Transgenic research · 2013Review
- Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection.Plant cell reports · 2013Article
- Citrus tristeza virus p23: a unique protein mediating key virus-host interactions.Frontiers in microbiology · 2013Article
- Genetic transformation of fruit trees: current status and remaining challenges.Transgenic research · 2012Review
- Role of RNA interference in plant improvement.Die Naturwissenschaften · 2011Review
- Citrus tristeza virus infection induces the accumulation of viral small RNAs (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile.Plant molecular biology · 2011Article
- Testing the 3'-untranslated RNA regions of beet necrotic yellow vein virus and beet yellows virus as inducers of posttranscriptional gene silencing.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mexican lime plants transformed with the 3'-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgene-derived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations from all sense, antisense and empty-vector transgenic lines were susceptible to CTV, except for a single sense-line plant with a complex transgene integration pattern that showed transgene-derived siRNAs in association with low levels of the transgene-derived transcript. In contrast, nine of 30 intron-hairpin lines showed CTV resistance, with 9%-56% of bud-propagated plants, depending on the line, remaining uninfected on graft inoculation, and the others being susceptible. Although resistance was always associated with the presence of transgene-derived siRNAs, their level in different sense and intron-hairpin transformants was variable irrespective of the response to CTV infection. In intron-hairpin lines with single transgene integration, CTV resistance was correlated with low accumulation of the transgene-derived transcript rather than with high accumulation of transgene-derived siRNAs.
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