ArticleFunctional & integrative genomics2010
Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa.
Article in Functional & integrative genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 104 citations in OpenAlex.
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- Direct shoot regeneration and gene expression profiling for optimized picroside-I biosynthesis in Picrorhiza Kurroa.Scientific reports · 2025Article
- A Comprehensive Analysis of Transcriptomics and Metabolomics Reveals Key Genes Involved in Terpenes Biosynthesis Pathway ofInternational journal of molecular sciences · 2025Article
- Varying levels of natural light intensity affect the phyto-biochemical compounds, antioxidant indices and genes involved in the monoterpene biosynthetic pathway of Origanum majorana L.BMC plant biology · 2024Article
- The Relationship between Endophytic Fungi ofJournal of fungi (Basel, Switzerland) · 2024Article
- Light regulation of the biosynthesis of phenolics, terpenoids, and alkaloids in plants.Communications biology · 2023Review
- Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis inPlants (Basel, Switzerland) · 2023Article
- Impacts of Different Light Spectra on CBD, CBDA and Terpene Concentrations in Relation to the Flower Positions of DifferentPlants (Basel, Switzerland) · 2022Article
- Effects of Light on Secondary Metabolite Biosynthesis in Medicinal Plants.Frontiers in plant science · 2021Review
- Stress-ResponsiveFrontiers in plant science · 2021Article
- Production of Encecalin in Cell Cultures and Hairy Roots ofMolecules (Basel, Switzerland) · 2020Article
- Two light responsive3 Biotech · 2020Article
- Characterization and functional analysis of two novel 3-hydroxy-3-methylglutaryl-coenzyme A reductase genes (GbHMGR2 and GbHMGR3) from Ginkgo biloba.Scientific reports · 2019Article
- Metabolomic understanding of intrinsic physiology inJournal of ginseng research · 2019Article
- Comparative transcriptomics uncovers differences in photoautotrophic versus photoheterotrophic modes of nutrition in relation to secondary metabolites biosynthesis in Swertia chirayita.Molecular biology reports · 2018Article
- Modular Design of Picroside-II Biosynthesis Deciphered through NGS Transcriptomes and Metabolic Intermediates Analysis in Naturally Variant Chemotypes of a Medicinal Herb,Frontiers in plant science · 2017Article
- Comparative transcriptome analysis in different tissues of a medicinal herb, Picrorhiza kurroa pinpoints transcription factors regulating picrosides biosynthesis.Molecular biology reports · 2016Article
- Discerning picroside-I biosynthesis via molecular dissection of in vitro shoot regeneration in Picrorhiza kurroa.Plant cell reports · 2016Article
- Article
- Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications.3 Biotech · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Picrorhiza (Picrorhiza kurrooa) is an endangered medicinal plant with well-known hepatoprotective activity attributed to monoterpenoid picrosides. The present article details on regulatory genes of terpenoid metabolism, 3-hydroxy-3-methylglutaryl coenzyme A reductase (pkhmgr) and 1-deoxy-D-xylulose-5-phosphate synthase (pkdxs) from picrorhiza. Since no molecular information was available, these genes were cloned to full-length by degenerate primers and rapid amplification of cDNA ends, followed by cloning of the upstream sequences that showed the presence of core sequences for light and temperature responsiveness. Electrophoretic mobility shift assay confirmed binding of protein to these motifs. Expression of pkhmgr and pkdxs was up-regulated at 15 degrees C as compared to at 25 degrees C as well as under light as compared to dark conditions. Picrosides content exhibited the trend similar to gene expression. To rule out the possible limitation of carbon pool under dark condition, plantlets of picrorhiza were raised in vitro in Murashige and Skoog medium supplemented with 3% sucrose. Results showed similar up-regulation of both the genes and the higher picrosides content in in vitro raised plantlets in the presence of light. Data suggested the important roles played by light and temperature in regulating pkhmgr and pkdxs, and the picrosides level in picrorhiza.
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