ArticleThe Journal of cell biology1991
The CaaX motif is required for isoprenylation, carboxyl methylation, and nuclear membrane association of lamin B2.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 199 citations in OpenAlex.
- Dynamic Post-translational Modifications of Nuclear Lamins in Cellular Function and Pathophysiology.Sub-cellular biochemistry · 2025Review
- SUN4 is a spermatid type II inner nuclear membrane protein that forms heteromeric assemblies with SUN3 and interacts with lamin B3.Journal of cell science · 2023Article
- A Nuclear Belt Fastens on Neural Cell Fate.Cells · 2022Review
- Nuclear Lamins: Key Proteins for Embryonic Development.Biology · 2022Review
- Post-Translational Modification of Lamins: Mechanisms and Functions.Frontiers in cell and developmental biology · 2022Review
- Drosophila p38 MAPK interacts with BAG-3/starvin to regulate age-dependent protein homeostasis.Aging cell · 2021Article
- Functional mechanisms and abnormalities of the nuclear lamina.Nature cell biology · 2021Review
- From structural resilience to cell specification - Intermediate filaments as regulators of cell fate.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Review
- Polyisoprenylated Cysteinyl Amide Inhibitors: A Novel Approach to Controlling Cancers with Hyperactive Growth Signaling.Current medicinal chemistry · 2021Article
- Lamin A/C Mechanotransduction in Laminopathies.Cells · 2020Review
- Beyond the Mevalonate Pathway: Control of Post-Prenylation Processing by Mutant p53.Frontiers in oncology · 2020Review
- Mechanisms of allelic and clinical heterogeneity of lamin A/C phenotypes.Physiological genomics · 2018Review
- Review
- Non-senescentAging · 2018Article
- Efficient farnesylation of an extended C-terminal C(The Journal of biological chemistry · 2018Article
- Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance.Genes & development · 2017Article
- Non-farnesylated B-type lamin can tether chromatin inside the nucleus and its chromatin interaction requires the Ig-fold region.Chromosoma · 2017Article
- Hsp70/J-protein machinery from Glossina morsitans morsitans, vector of African trypanosomiasis.PloS one · 2017Article
- Article
- BioID Identification of Lamin-Associated Proteins.Methods in enzymology · 2016Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent evidence suggests that the conserved COOH-terminal CaaX motif of nuclear lamins may play a role in targeting newly synthesized proteins to the nuclear envelope. We have shown previously that in rabbit reticulocyte lysates the cysteine residue of the CaaX motif of chicken lamin B2 is necessary for incorporation of a derivative of mevalonic acid, the precursor of isoprenoids. Here we have analyzed the properties of normal and mutated forms of chicken lamin B2 stably expressed in mouse L cells. Mutation of the cysteine residue of the CaaX motif to alanine or introduction of a stop codon immediately after the cysteine residue was found to abolish both isoprenylation and carboxyl methylation of transfected lamin B2. Concomitantly, although nuclear import of the mutant lamin B2 proteins was preserved, their association with the inner nuclear membrane was severely impaired. From these results we conclude that the COOH-terminal CaaX motif is required for isoprenylation and carboxyl methylation of lamins in vivo, and that these modifications are important for association of B-type lamins with the nucleoplasmic surface of the inner nuclear membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.