ArticleThe American journal of pathology2010
BRCA1 protein and nucleolin colocalize in breast carcinoma tissue and cancer cell lines.
Article in The American journal of pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Identification of Hub of the Hub-Genes From Different Individual Studies for Early Diagnosis, Prognosis, and Therapies of Breast Cancer.Bioinformatics and biology insights · 2024Article
- Changes in Nucleolin Expression during Malignant Transformation Leading to Ovarian High-Grade Serous Carcinoma.Cancers · 2023Article
- The BRCA1 BRCT promotes antisense RNA production and double-stranded RNA formation to suppress ribosomal R-loops.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Nucleolin promotes Ang II‑induced phenotypic transformation of vascular smooth muscle cells via interaction with tropoelastin mRNA.International journal of molecular medicine · 2019Article
- Synthesis and evaluation of anJournal of labelled compounds & radiopharmaceuticals · 2016Article
- S100A14, a member of the EF-hand calcium-binding proteins, is overexpressed in breast cancer and acts as a modulator of HER2 signaling.The Journal of biological chemistry · 2014Article
- Localization of BRCA1 protein in breast cancer tissue and cell lines with mutations.Cancer cell international · 2013Article
- Genome wide proteomics of ERBB2 and EGFR and other oncogenic pathways in inflammatory breast cancer.Journal of proteome research · 2013Article
- Nucleic Acid Aptamers as Potential Therapeutic and Diagnostic Agents for Lymphoma.Journal of cancer therapy · 2013Article
- Quantitative analysis of BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer tissue.PloS one · 2013Article
- Proline-mediated proteasomal degradation of the prostate-specific tumor suppressor NKX3.1.The Journal of biological chemistry · 2012Article
- The DNA damage effector Chk1 kinase regulates Cdc14B nucleolar shuttling during cell cycle progression.Cell cycle (Georgetown, Tex.) · 2011Article
- Extracellular signal-regulated kinases modulate DNA damage response - a contributing factor to using MEK inhibitors in cancer therapy.Current medicinal chemistry · 2011Review
- The Expression Patterns of ER, PR, HER2, CK5/6, EGFR, Ki-67 and AR by Immunohistochemical Analysis in Breast Cancer Cell Lines.Breast cancer : basic and clinical research · 2010Article
- The Nucleolus Takes Control of Protein Trafficking Under Cellular Stress.Molecular and cellular pharmacology · 2010Article
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
Abstract
The breast and ovarian cancer susceptibility gene BRCA1 encodes a tumor suppressor. BRCA1 protein, which is involved in DNA damage response, has been thought to be found primarily in cell nuclei. In the present investigation, immunohistological studies of BRCA1 protein in frozen breast cancer tissue and MCF7 and HeLa cell lines revealed BRCA1 expression in both nucleoli and nucleoplasmic foci. Immunoelectron microscopic studies of estrogen-stimulated MCF7 cells demonstrated BRCA1 protein localization in the granular components of the nucleolus. Moreover, immunofluorescence of BRCA1 and nucleolin double-labeling showed colocalization in both nucleoli and nucleoplasmic foci in breast tumor cells and asynchronously growing MCF7 and HeLa cells. Multiparameter analysis of BRCA1 and nucleolin in relation to cell cycle position (DNA content) showed expression during G1-S and persistence of BRCA1 during G2/M. After gamma-irradiation of MCF7 cells, BRCA1 protein dispersed from nucleoli and nucleoplasmic foci to other nucleoplasmic sites, which did not colocalize with nucleolin. Small interfering RNA-mediated knockdown of BRCA1 protein resulted in decreased immunofluorescence staining, which was confirmed by Western blotting. The observed colocalization of BRCA1 and nucleolin raises new possibilities for the nucleoplasm-nucleolus pathways of these proteins and their functional significance.
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Registered trials
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