ArticleEnvironmental microbiology2010
Cultivation of a Synergistetes strain representing a previously uncultivated lineage.
Article in Environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Differences in the subgingival microbial population of chronic periodontitis in subjects with and without type 2 diabetes mellitus-a systematic review.Clinical oral investigations · 2018Pooled it
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- Deciphering the role of female reproductive tract microbiome in reproductive health: a review.Frontiers in cellular and infection microbiology · 2024Review
- Long-term label-free assessments of individual bacteria using three-dimensional quantitative phase imaging and hydrogel-based immobilization.Scientific reports · 2023Article
- Isolation, identification, and significance of salivaryFrontiers in cellular and infection microbiology · 2023Article
- Advances in the oral microbiota and rapid detection of oral infectious diseases.Frontiers in microbiology · 2023Review
- Identification of a growth factor required for culturing specific fastidious oral bacteria.Journal of oral microbiology · 2023Article
- A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro.Biotech (Basel (Switzerland)) · 2022Article
- Effectiveness of decontamination protocols when analyzing ancient DNA preserved in dental calculus.Scientific reports · 2021Article
- Dysbiosis of Oral Microbiota Associated with Palmoplantar Pustulosis.Dermatology (Basel, Switzerland) · 2021Observational
- Establishing a mucosal gut microbial community in vitro using an artificial simulator.PloS one · 2018Article
- A practical guide to the oral microbiome and its relation to health and disease.Oral diseases · 2017Review
- Draft genome sequence ofStandards in genomic sciences · 2017Article
- Cultivation strategies for growth of uncultivated bacteria.Journal of oral biosciences · 2016Article
- First Cultivation of Health-Associated Tannerella sp. HOT-286 (BU063).Journal of dental research · 2016Article
- A Flow Cytometry Method for Rapidly Assessing Mycobacterium tuberculosis Responses to Antibiotics with Different Modes of Action.Antimicrobial agents and chemotherapy · 2016Article
- In Vitro Cultivation of 'Unculturable' Oral Bacteria, Facilitated by Community Culture and Media Supplementation with Siderophores.PloS one · 2016Article
- In vitro culture of previously uncultured oral bacterial phylotypes.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Subgingival plaque samples obtained from human subjects with periodontitis, shown to include previously uncultivable members of the phylum Synergistetes, were used to inoculate Cooked Meat Medium (CMM). The presence of Cluster A (uncultivable) Synergistetes was monitored by fluorescent in situ hybridization (FISH) and quantitative PCR (Q-PCR). Cluster A Synergistetes were found to grow in CMM in co-culture with other plaque bacteria and growth was stimulated by the addition of mucin and serum. Plaque samples were also used to inoculate Blood Agar (BA) plates and growth of Cluster A Synergistetes was revealed after anaerobic incubation, by colony hybridization with specific probes. Surface growth on the plates in regions identified by colony hybridization was harvested and used to inoculate fresh plates, thus enriching for Cluster A Synergistetes. Cross-streaks of other plaque bacteria were also used to stimulate Synergistetes growth. In the early passages, no discrete Synergistetes colonies were seen, but after eight passages and the use of cross-streaks of other bacteria present in the enriched community, colonies arose, which consisted solely of Cluster A Synergistetes cells, as determined by 16S rRNA gene PCR and cloning. This is the first report of the successful culture of a member of the uncultivable branch of this phylum.
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Registered trials
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