ArticleThe Australian and New Zealand journal of psychiatry2010
Selection of reference gene expression in a schizophrenia brain cohort.
Article in The Australian and New Zealand journal of psychiatry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 108 citations in OpenAlex.
- Increased Tumor Necrosis Factor Superfamily Members in Neuroinflammatory Schizophrenia and Bipolar Disorder Midbrains.Biological psychiatry global open science · 2026Article
- Effect of Testosterone on Maintenance of Morphine-induced Conditioned Place Preference: Androgen and μ-opioid Receptor Gene Expressions in PFC and NAc of Rats.Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology · 2026Article
- Transcriptional evidence of reduced BDNF trophic capacity in the post-mortem human midbrain of schizophrenia cases with high inflammation.Translational psychiatry · 2025Article
- Cytomegalovirus antibodies are associated with mood disorders, suicide, markers of neuroinflammation, and microglia activation in postmortem brain samples.Molecular psychiatry · 2023Article
- The influence of tissue pH and RNA integrity number on gene expression of human postmortem brain.Frontiers in psychiatry · 2023Article
- Evidence for increased DNA damage repair in the postmortem brain of the high stress-response group of schizophrenia.Frontiers in psychiatry · 2023Article
- Increased levels of a pro-inflammatory IgG receptor in the midbrain of people with schizophrenia.Journal of neuroinflammation · 2022Article
- Expression Analysis of Ermin and Listerin E3 Ubiquitin Protein Ligase 1 Genes in the Periphery of Patients with Schizophrenia.Journal of molecular neuroscience : MN · 2022Article
- Increased levels of midbrain immune-related transcripts in schizophrenia and in murine offspring after maternal immune activation.Molecular psychiatry · 2021Article
- Nuclear factor kappa B activation appears weaker in schizophrenia patients with high brain cytokines than in non-schizophrenic controls with high brain cytokines.Journal of neuroinflammation · 2020Article
- Increased macrophages and changed brain endothelial cell gene expression in the frontal cortex of people with schizophrenia displaying inflammation.Molecular psychiatry · 2020Article
- Evidence for Decreased Density of Calretinin-Immunopositive Neurons in the Caudate Nucleus in Patients With Schizophrenia.Frontiers in neuroanatomy · 2020Article
- Schizophrenia-associated MicroRNA-Gene Interactions in the Dorsolateral Prefrontal Cortex.Genomics, proteomics & bioinformatics · 2019Article
- Human Brain Region-Specific Alternative Splicing of TRPC3, the Type 3 Canonical Transient Receptor Potential Non-Selective Cation Channel.Cerebellum (London, England) · 2019Article
- Circular RNA biogenesis is decreased in postmortem cortical gray matter in schizophrenia and may alter the bioavailability of associated miRNA.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2019Article
- Elevated ubiquitinated proteins in brain and blood of individuals with schizophrenia.Scientific reports · 2019Article
- Nuclear Receptors and Neuroinflammation in Schizophrenia.Molecular neuropsychiatry · 2018Article
- A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia.Journal of psychiatry & neuroscience : JPN · 2018Article
- Altered Expression Profile of IgLON Family of Neural Cell Adhesion Molecules in the Dorsolateral Prefrontal Cortex of Schizophrenic Patients.Frontiers in molecular neuroscience · 2018Article
- Using blood cytokine measures to define high inflammatory biotype of schizophrenia and schizoaffective disorder.Journal of neuroinflammation · 2017Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveIn order to conduct postmortem human brain research into the neuropatho-logical basis of schizophrenia, it is critical to establish cohorts that are well-characterized and well-matched. The aim of the present study was therefore to determine if specimen characteristics including: diagnosis, age, postmortem interval (PMI), brain acidity (pH), and/or the agonal state of the subject at death related to RNA quality, and to determine the most appropriate reference gene mRNAs.
methodsA matched cohort was selected of 74 subjects (schizophrenia/schizoaffective disorder, n = 37; controls, n = 37). Middle frontal gyrus tissue was pulverized, tissue pH was measured, RNA isolated for cDNA from each case, and RNA integrity number (RIN) measurements were assessed. Using quantitative reverse transcription-polymerase chain reaction, nine housekeeper genes were measured and a geomean calculated per case in each diagnostic group.
resultsThe RINs were very good (mean = 7.3) and all nine housekeeper control genes were significantly correlated with RIN. Seven of nine housekeeper genes were also correlated with pH; two clinical variables, agonal state and duration of illness, did have an effect on some control mRNAs. No major impact of PMI or freezer time on housekeeper mRNAs was detected. The results show that people with schizophrenia had significantly less PPIA and SDHA mRNA and tended to have less GUSB and B2M mRNA, suggesting that these control genes may not be good candidates for normalization.
conclusionsIn the present cohort <10% variability in RINs was detected and the diagnostic groups were well matched overall. The cohort was adequately powered (0.80-0.90) to detect mRNA differences (25%) due to disease. The study suggests that multiple factors should be considered in mRNA expression studies of human brain tissues. When schizophrenia cases are adequately matched to control cases subtle differences in gene expression can be reliably detected.
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