Evidence map›Paper›PMID 20072916›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2010

Multiphoton redox ratio imaging for metabolic monitoring in vivo.

Melissa Skala, Nirmala Ramanujam

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 93 citations in OpenAlex.

  1. Article
  2. Fluorescence lifetime imaging ophthalmoscopy (FLIO) of patients with neovascular age-related macular degeneration before and after treatment with intravitreal ranibizumab.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2026
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  6. Review
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  10. Metabolic changes to host cells withbioRxiv : the preprint server for biology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Melissa SkalaDepartment of Biomedical Engineering, Duke University, CIEMAS, Durham, NC, USA. melissa.skala@duke.edu
Nirmala Ramanujam
Duke University · US

Funding

A Spectral/Lifetime Microscope for in vivo StudiesR01EB000184 · NIBIB · UNIVERSITY OF WISCONSIN-MADISON · PI WHITE, JOHN GRAHAM · 2002 to 2006
$1.9M
Molecular optical coherence tomography for monitoring cancer therapyF32CA130309 · NCI · DUKE UNIVERSITY · PI SKALA, MELISSA CAROLINE · 2007 to 2009
$110k
Functional Optical Coherence Tomography for Monitoring Drug Resistance in CancerK99CA142888 · NCI · DUKE UNIVERSITY · PI SKALA, MELISSA CAROLINE · 2009 to 2009
$89k
NCI NIH HHS F32 CA130309NCI NIH HHS K99 CA142888NIBIB NIH HHS R01 EB000184
6 · The paper itself

Abstract

Metabolic monitoring at the cellular level in live tissues is important for understanding cell function, disease processes, and potential therapies. Multiphoton imaging of the relative amounts of NADH and FAD (the primary electron donor and acceptor, respectively, in the electron transport chain) provides a noninvasive method for monitoring cellular metabolic activity with high resolution in three dimensions in vivo. NADH and FAD are endogenous tissue fluorophores, and thus this method does not require exogenous stains or tissue excision. We describe the principles and protocols of multiphoton redox ratio imaging in vivo.

Indexed as

AnimalsFlavin-Adenine DinucleotideMiceMicroscopy, Fluorescence, MultiphotonMitochondriaNADOxidation-ReductionFlavin-Adenine DinucleotideNAD

Identifiers

PMID20072916
PMCPMC2874879
OpenAlexW1587705295

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.