ArticlePloS one2010
Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- Unveiling of phytochemicals, antioxidants, cytotoxic effects and anthelmintic potency of green tea (Camellia sinensis) beverage against albendazole resistant Hamonchus contortus.Scientific reports · 2026Article
- Catechin gallate triggers metabolomic and lipidomic alteration in Toxoplasma gondii.Parasites & vectors · 2025Article
- Contemporary exploitation of natural products for arthropod-borne pathogen transmission-blocking interventions.Parasites & vectors · 2022Review
- The Role of Hsp70s in the Development and Pathogenicity of Plasmodium falciparum.Advances in experimental medicine and biology · 2021Article
- In Vitro Growth- and Encystation-Inhibitory Efficacies of Matcha Green Tea and Epigallocatechin Gallate AgainstPathogens (Basel, Switzerland) · 2020Article
- Anti-Toxoplasma activity of Sorghum bicolor-derived lipophilic fractions.BMC research notes · 2019Article
- Screening for potential prophylactics targeting sporozoite motility through the skin.Malaria journal · 2018Article
- Developing transmission-blocking strategies for malaria control.PLoS pathogens · 2017Review
- Progress in imaging methods: insights gained into Plasmodium biology.Nature reviews. Microbiology · 2017Review
- Biophysical characteristics of proteins and living cells exposed to the green tea polyphenol epigallocatechin-3-gallate (EGCg): review of recent advances from molecular mechanisms to nanomedicine and clinical trials.European biophysics journal : EBJ · 2017Review
- The Effect of Co-Administration of Death Camas (Zigadenus spp.) and Low Larkspur (Delphinium spp.) in Cattle.Toxins · 2016Article
- Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol.Molecules (Basel, Switzerland) · 2015Article
- Combinations of alkaloids affecting different molecular targets with the saponin digitonin can synergistically enhance trypanocidal activity against Trypanosoma brucei brucei.Antimicrobial agents and chemotherapy · 2015Article
- Inhibitory effect of novel iron chelator, 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) and green tea extract on growth of Plasmodium falciparum.Malaria journal · 2015Article
- Modes of Action of Herbal Medicines and Plant Secondary Metabolites.Medicines (Basel, Switzerland) · 2015Review
- Active migration and passive transport of malaria parasites.Trends in parasitology · 2015Review
- Toxicity and Loss of Mitochondrial Membrane Potential Induced by Alkyl Gallates in Trypanosoma cruzi.International scholarly research notices · 2015Article
- Medicinal plants: a source of anti-parasitic secondary metabolites.Molecules (Basel, Switzerland) · 2012Review
- Environmental constraints guide migration of malaria parasites during transmission.PLoS pathogens · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMost medicinal plants contain a mixture of bioactive compounds, including chemicals that interact with intracellular targets and others that can act as adjuvants to facilitate absorption of polar agents across cellular membranes. However, little is known about synergistic effects between such potential drug candidates and adjuvants. To probe for such effects, we tested the green tea compound epigallocatechin gallate (EGCG) and the membrane permeabilising digitonin on Plasmodium sporozoite motility and viability. METHODOLOGY/PRINCIPAL
findingsGreen fluorescent P. berghei sporozoites were imaged using a recently developed visual screening methodology. Motility and viability parameters were automatically analyzed and IC50 values were calculated, and the synergism of drug and adjuvant was assessed by the fractional inhibitory concentration index. Validating our visual screening procedure, we showed that sporozoite motility and liver cell infection is inhibited by EGCG at nontoxic concentrations. Digitonin synergistically increases the cytotoxicity of EGCG on sporozoite survival, but shows an additive effect on sporozoite motility. CONCLUSIONS/SIGNIFICANCE: We proved the feasibility of performing highly reliable visual screens for compounds against Plasmodium sporozoites. We thereby could show an advantage of administering mixtures of plant metabolites on inhibition of cell motility and survival. Although the effective concentration of both drugs is too high for use in malaria prophylaxis, the demonstration of a synergistic effect between two plant compounds could lead to new avenues in drug discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.