ArticleNucleic acids research2010
Efficient transcription through an intron requires the binding of an Sm-type U1 snRNP with intact stem loop II to the splice donor.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Gene augmentation for autosomal dominant retinitis pigmentosa using rhodopsin genomic loci nanoparticles in the P23HGene therapy · 2023Article
- A novel approach to analyze the association characteristics between post-spliced introns and their corresponding mRNA.Frontiers in genetics · 2023Article
- Prospects of Non-Coding Elements in Genomic DNA Based Gene Therapy.Current gene therapy · 2022Review
- Multiply spliced HIV RNA is a predictive measure of virus production ex vivo and in vivo following reversal of HIV latency.EBioMedicine · 2021Article
- HIV latency reversing agents act through Tat post translational modifications.Retrovirology · 2018Article
- Therapeutic activity of modified U1 core spliceosomal particles.Nature communications · 2016Article
- Improvement of SMN2 pre-mRNA processing mediated by exon-specific U1 small nuclear RNA.American journal of human genetics · 2015Article
- Alternative splicing acting as a bridge in evolution.Stem cell investigation · 2015Article
- HIV-1 transcription is regulated by splicing factor SRSF1.Nucleic acids research · 2014Article
- Hyperimmune bovine colostrum as a low-cost, large-scale source of antibodies with broad neutralizing activity for HIV-1 envelope with potential use in microbicides.Antimicrobial agents and chemotherapy · 2012Article
- Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion.Nature communications · 2012Article
- Co-expression of miRNA targeting the expression of PERK, but not PKR, enhances cellular immunity from an HIV-1 Env DNA vaccine.PloS one · 2011Article
- Excessive RNA splicing and inhibition of HIV-1 replication induced by modified U1 small nuclear RNAs.Journal of virology · 2010Article
- Where splicing joins chromatin.Nucleus (Austin, Tex.)Review
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Authors and funding
4 authors.
Funding
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Abstract
The mechanism behind the positive action of introns upon transcription and the biological significance of this positive feedback remains unclear. Functional ablation of splice sites within an HIV-derived env cDNA significantly reduced transcription that was rescued by a U1 snRNA modified to bind to the mutated splice donor (SD). Using this model we further characterized both the U1 and pre-mRNA structural requirements for transcriptional enhancement. U1 snRNA rescued as a mature Sm-type snRNP with an intact stem loop II. Position and sequence context for U1-binding is crucial because a promoter proximal intron placed upstream of the mutated SD failed to rescue transcription. Furthermore, U1-rescue was independent of promoter and exon sequence and is partially replaced by the transcription elongation activator Tat, pointing to an intron-localized block in transcriptional elongation. Thus, transcriptional coupling of U1 snRNA binding to the SD may licence the polymerase for transcription through the intron.
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