ArticleNucleic acids research2010
A putative mobile genetic element carrying a novel type IIF restriction-modification system (PluTI).
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Bacterial restriction-modification systems: mechanisms of defense against phage infection.Biophysics reports · 2025Article
- Base-excision restriction enzymes: expanding the world of epigenetic immune systems.DNA research : an international journal for rapid publication of reports on genes and genomes · 2023Article
- Evolution of Immune Systems From Viruses and Transposable Elements.Frontiers in microbiology · 2019Review
- The complete methylome of an entomopathogenic bacterium reveals the existence of loci with unmethylated Adenines.Scientific reports · 2018Article
- Silencing of cryptic prophages in Corynebacterium glutamicum.Nucleic acids research · 2016Article
- Type II restriction endonucleases--a historical perspective and more.Nucleic acids research · 2014Review
- Comparative genomics of defense systems in archaea and bacteria.Nucleic acids research · 2013Review
- The phn Island: A New Genomic Island Encoding Catabolism of Polynuclear Aromatic Hydrocarbons.Frontiers in microbiology · 2012Article
- Defense islands in bacterial and archaeal genomes and prediction of novel defense systems.Journal of bacteriology · 2011Article
- IS-linked movement of a restriction-modification system.PloS one · 2011Article
- Conflicts targeting epigenetic systems and their resolution by cell death: novel concepts for methyl-specific and other restriction systems.DNA research : an international journal for rapid publication of reports on genes and genomes · 2010Review
- Genome comparison and context analysis reveals putative mobile forms of restriction-modification systems and related rearrangements.Nucleic acids research · 2010Article
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Authors and funding
7 authors at 1 institution in 3 countries.
Funding
Abstract
Genome comparison and genome context analysis were used to find a putative mobile element in the genome of Photorhabdus luminescens, an entomopathogenic bacterium. The element is composed of 16-bp direct repeats in the terminal regions, which are identical to a part of insertion sequences (ISs), a DNA methyltransferase gene homolog, two genes of unknown functions and an open reading frame (ORF) (plu0599) encoding a protein with no detectable sequence similarity to any known protein. The ORF (plu0599) product showed DNA endonuclease activity, when expressed in a cell-free expression system. Subsequently, the protein, named R.PluTI, was expressed in vivo, purified and found to be a novel type IIF restriction enzyme that recognizes 5'-GGCGC/C-3' (/ indicates position of cleavage). R.PluTI cleaves a two-site supercoiled substrate at both the sites faster than a one-site supercoiled substrate. The modification enzyme homolog encoded by plu0600, named M.PluTI, was expressed in Escherichia coli and shown to protect DNA from R.PluTI cleavage in vitro, and to suppress the lethal effects of R.PluTI expression in vivo. These results suggested that they constitute a restriction-modification system, present on the putative mobile element. Our approach thus allowed detection of a previously uncharacterized family of DNA-interacting proteins.
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Registered trials
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