ArticleNucleic acids research2010
Functional cooperation between CREM and GCNF directs gene expression in haploid male germ cells.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Molecular Characterization of the Murine Catsper4 Promoter and its Regulation by CREMτ.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Novel Aspects of cAMP-Response Element Modulator (CREM) Role in Spermatogenesis and Male Fertility.International journal of molecular sciences · 2023Review
- Research progress in the role and mechanism of Leucine in regulating animal growth and development.Frontiers in physiology · 2023Review
- Characterization of the promoter region of the murine Catsper2 gene.FEBS open bio · 2022Article
- microRNA-196a-5p inhibits testicular germ cell tumor progression via NR6A1/E-cadherin axis.Cancer medicine · 2020Article
- Article
- The arginine methyltransferase CARM1 represses p300•ACT•CREMτ activity and is required for spermiogenesis.Nucleic acids research · 2018Article
- Positive expression of NR6A1/CT150 as a predictor of biochemical recurrence-free survival in prostate cancer patients.Oncotarget · 2017Article
- SLE-Associated Defects Promote Altered T Cell Function.Critical reviews in immunology · 2017Review
- A 5'-flanking region of gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) gene directs its cell-specific androgen-regulated gene expression in testicular germ cells.Endocrinology · 2013Article
- cAMP responsive element modulator: a critical regulator of cytokine production.Trends in molecular medicine · 2013Review
- Identification and characterization of promoter and regulatory regions for mouse Adam2 gene expression.Molecular biology reports · 2013Article
- Transcriptional regulation of the hypocretin/orexin gene by NR6A1.Biochemical and biophysical research communications · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular differentiation and development of germ cells critically depend on a coordinated activation and repression of specific genes. The underlying regulation mechanisms, however, still lack a lot of understanding. Here, we describe that both the testis-specific transcriptional activator CREMtau (cAMP response element modulator tau) and the repressor GCNF (germ cell nuclear factor) have an overlapping binding site which alone is sufficient to direct cell type-specific expression in vivo in a heterologous promoter context. Expression of the transgene driven by the CREM/GCNF site is detectable in spermatids, but not in any somatic tissue or at any other stages during germ cell differentiation. CREMtau acts as an activator of gene transcription whereas GCNF suppresses this activity. Both factors compete for binding to the same DNA response element. Effective binding of CREM and GCNF highly depends on composition and epigenetic modification of the binding site. We also discovered that CREM and GCNF bind to each other via their DNA binding domains, indicating a complex interaction between the two factors. There are several testis-specific target genes that are regulated by CREM and GCNF in a reciprocal manner, showing a similar activation pattern as during spermatogenesis. Our data indicate that a single common binding site for CREM and GCNF is sufficient to specifically direct gene transcription in a tissue-, cell type- and differentiation-specific manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.