ArticleInvestigative ophthalmology & visual science2010
Hydrostatic pressure-induced release of stored calcium in cultured rat optic nerve head astrocytes.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Advancing In Vitro Microfluidic Models for Pressure-Induced Retinal Ganglion Cell Degeneration: Current Insights and Future Directions from a Biomechanical Perspective.Micromachines · 2025Article
- Elevated hydrostatic pressure destabilizes VE-cadherin junctions in a time and shear stress dependent manner: An endothelium-on-chip study.APL bioengineering · 2025Article
- Emulating clinical pressure waveforms in cell culture using an Arduino-controlled millifluidic 3D-printed platform for 96-well plates.Lab on a chip · 2023Article
- A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System.International journal of molecular sciences · 2022Review
- Article
- Mechanosensitive channel inhibition attenuates TGFβ2-induced actin cytoskeletal remodeling and reactivity in mouse optic nerve head astrocytes.Experimental eye research · 2021Article
- Looking for In Vitro Models for Retinal Diseases.International journal of molecular sciences · 2021Review
- Molecular cancer cell responses to solid compressive stress and interstitial fluid pressure.Cytoskeleton (Hoboken, N.J.) · 2021Review
- A lab-on-a-chip model of glaucoma.Brain and behavior · 2020Article
- Regulation of Cell Behavior by Hydrostatic Pressure.Applied mechanics reviews · 2019Review
- Relationship between Sigma-1 receptor and BDNF in the visual system.Experimental eye research · 2018Article
- Article
- Improvement of In Vitro Three-Dimensional Cartilage Regeneration by a Novel Hydrostatic Pressure Bioreactor.Stem cells translational medicine · 2017Article
- Sigma 1 receptor regulates ERK activation and promotes survival of optic nerve head astrocytes.PloS one · 2017Article
- Plate reader-based cell viability assays for glioprotection using primary rat optic nerve head astrocytes.Experimental eye research · 2015Article
- Calcium entry via connexin hemichannels in lens epithelium.Experimental eye research · 2015Article
- Differential subcellular Ca2+ signaling in a highly specialized subpopulation of astrocytes.Experimental neurology · 2015Article
- Astrocytes in the optic nerve head express putative mechanosensitive channels.Molecular vision · 2015Article
- Integration of modeling with experimental and clinical findings synthesizes and refines the central role of inositol 1,4,5-trisphosphate receptor 1 in spinocerebellar ataxia.Frontiers in neuroscience · 2014Review
- TRPV4 in porcine lens epithelium regulates hemichannel-mediated ATP release and Na-K-ATPase activity.American journal of physiology. Cell physiology · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeElevated intraocular pressure is associated with glaucomatous optic nerve damage. Other investigators have shown functional changes in optic nerve head astrocytes subjected to elevated hydrostatic pressure (HP) for 1 to 5 days. Recently, the authors reported ERK1/2, p90(RSK) and NHE1 phosphorylation after 2 hours. Here they examine calcium responses at the onset of HP to determine what precedes ERK1/2 phosphorylation.
methodsCytoplasmic calcium concentration ([Ca(2+)](i)) was measured in cultured rat optic nerve astrocytes loaded with fura-2. The cells were placed in a closed imaging chamber and subjected to an HP increase of 15 mm Hg. Protein phosphorylation was detected by Western blot analysis.
resultsThe increase of HP caused an immediate slow increase in [Ca(2+)](i). The response persisted in calcium-free solution and when nickel chloride (4 mM) was added to suppress channel-mediated calcium entry. Previous depletion of the ER calcium stores by cyclopiazonic acid abolished the HP-induced calcium level increase. The HP-induced increase persisted in cells exposed to xestospongin C, an inhibitor of IP3R-mediated calcium release. In contrast, ryanodine receptor (RyR) antagonist ruthenium red (10 microM) or dantrolene (25 microM) inhibited the HP-induced calcium increase. The HP-induced calcium increase was abolished when ryanodine-sensitive calcium stores were pre-depleted with caffeine (3 mM). HP caused ERK1/2 phosphorylation. The magnitude of the ERK1/2 phosphorylation response was reduced by ruthenium red and dantrolene.
conclusionsIncreasing HP causes calcium release from a ryanodine-sensitive cytoplasmic store and subsequent ERK1/2 activation. Calcium store release appears to be a required early step in the initial astrocyte response to an HP increase.
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