ArticlePlant physiology2010
Combined bimolecular fluorescence complementation and Forster resonance energy transfer reveals ternary SNARE complex formation in living plant cells.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Cryptochrome 1 promotes photomorphogenesis in Arabidopsis by displacing substrates from the COP1 ubiquitin ligase.The Plant journal : for cell and molecular biology · 2025Article
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024Review
- Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity.Journal of experimental botany · 2023Article
- Visualization of in vivo protein-protein interactions in plants.Journal of experimental botany · 2022Review
- SNARE Complexity in Arbuscular Mycorrhizal Symbiosis.Frontiers in plant science · 2020Article
- Interaction Between the SNARE SYP121 and the Plasma Membrane Aquaporin PIP2;7 Involves Different Protein Domains.Frontiers in plant science · 2020Article
- How to prove the existence of metabolons?Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2018Review
- The plant membrane surrounding powdery mildew haustoria shares properties with the endoplasmic reticulum membrane.Journal of experimental botany · 2017Article
- Key Components of Different Plant Defense Pathways Are Dispensable for Powdery Mildew Resistance of the ArabidopsisFrontiers in plant science · 2017Article
- Techniques for the Analysis of Protein-Protein Interactions in Vivo.Plant physiology · 2016Review
- Article
- Biotrophy at Its Best: Novel Findings and Unsolved Mysteries of the Arabidopsis-Powdery Mildew Pathosystem.The arabidopsis book · 2016Article
- FRET-FLIM applications in plant systems.Protoplasma · 2014Review
- Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Fine mapping and chromosome walking towards the Ror1 locus in barley (Hordeum vulgare L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Filamentous plant pathogen effectors in action.Nature reviews. Microbiology · 2013Review
- Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells.Frontiers in plant science · 2013Review
- Phosphorylation of HopQ1, a type III effector from Pseudomonas syringae, creates a binding site for host 14-3-3 proteins.Plant physiology · 2013Article
- Arabidopsis paired amphipathic helix proteins SNL1 and SNL2 redundantly regulate primary seed dormancy via abscisic acid-ethylene antagonism mediated by histone deacetylation.The Plant cell · 2013Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Various fluorophore-based microscopic methods, comprising Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC), are suitable to study pairwise interactions of proteins in living cells. The analysis of interactions between more than two protein partners using these methods, however, remains difficult. In this study, we report the successful application of combined BiFC-FRET-fluorescence lifetime imaging microscopy and BiFC-FRET-acceptor photobleaching measurements to visualize the formation of ternary soluble N-ethylmaleimide-sensitive factor attachment receptor complexes in leaf epidermal cells. This method expands the repertoire of techniques to study protein-protein interactions in living plant cells by a procedure capable of visualizing simultaneously interactions between three fluorophore-tagged polypeptide partners.
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