ArticleThe Journal of biological chemistry2010
Aquaporin-4 (AQP4) associations and array dynamics probed by photobleaching and single-molecule analysis of green fluorescent protein-AQP4 chimeras.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- In vivo imaging of the barrier properties of the glia limitans during health and neuroinflammation.Nature communications · 2025Article
- Article
- Review
- Article
- AQP4e-Based Orthogonal Arrays Regulate Rapid Cell Volume Changes in Astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Comparative molecular dynamics study of neuromyelitis optica-immunoglobulin G binding to aquaporin-4 extracellular domains.Biochimica et biophysica acta. Biomembranes · 2017Article
- Astrocyte Aquaporin Dynamics in Health and Disease.International journal of molecular sciences · 2016Review
- Subcellular functions of proteins under fluorescence single-cell microscopy.Biochimica et biophysica acta · 2016Review
- Aggregation state determines the localization and function of M1- and M23-aquaporin-4 in astrocytes.The Journal of cell biology · 2014Article
- Biology of AQP4 and anti-AQP4 antibody: therapeutic implications for NMO.Brain pathology (Zurich, Switzerland) · 2013Review
- Optic neuritis in neuromyelitis optica.Progress in retinal and eye research · 2013Article
- Article
- Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes.Journal of cell science · 2012Article
- Cell culture models and animal models for studying the patho-physiological role of renal aquaporins.Cellular and molecular life sciences : CMLS · 2012Review
- Small-molecule inhibitors of NMO-IgG binding to aquaporin-4 reduce astrocyte cytotoxicity in neuromyelitis optica.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Complement-dependent cytotoxicity in neuromyelitis optica requires aquaporin-4 protein assembly in orthogonal arrays.The Journal of biological chemistry · 2012Article
- Post-Golgi supramolecular assembly of aquaporin-4 in orthogonal arrays.Traffic (Copenhagen, Denmark) · 2012Article
- Live-cell imaging of aquaporin-4 supramolecular assembly and diffusion.Methods in enzymology · 2012Review
- Light inactivation of water transport and protein-protein interactions of aquaporin-Killer Red chimeras.The Journal of general physiology · 2012Article
- Hyperviscous airway periciliary and mucous liquid layers in cystic fibrosis measured by confocal fluorescence photobleaching.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The plasma membrane assembly of aquaporin-4 (AQP4) water channels into orthogonal arrays of particles (OAPs) involves interactions of AQP4 N-terminal domains. To study in live cells the site of OAP assembly, the size and dynamics of plasma membrane OAPs, and the heterotetrameric associations of AQP4, we constructed green fluorescent protein (GFP)-labeled AQP4 "long" (M1) and "short" (M23) isoforms in which GFP was inserted at the cytoplasm-facing N or C terminus or between Val-141 and Val-142 in the second extracellular loop of AQP4. The C-terminal and extracellular loop GFP insertions did not interfere with the rapid unrestricted membrane diffusion of GFP-labeled M1 or the restricted diffusion and OAP assembly of GFP-labeled M23. Photobleaching of brefeldin A-treated cells showed comparable and minimally restricted diffusion of M1 and M23, indicating that OAP assembly occurs post-endoplasmic reticulum. Single-molecule step photobleaching and intensity analysis of GFP-labeled M1 in the absence versus presence of excess unlabeled M1 or M23 with an OAP-disrupting mutation indicated heterotetrameric AQP4 association. Time-lapse total internal reflection fluorescence imaging of M23 in live cells at 37 degrees C indicated that OAPs diffuse slowly (D approximately 10(-12) cm(2)/s) and rearrange over tens of minutes. Our biophysical measurements in live cells thus reveal extensive AQP4 monomer-monomer and tetramer-tetramer interactions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.