ArticleThe Journal of biological chemistry2010
Initiation of purinergic signaling by exocytosis of ATP-containing vesicles in liver epithelium.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed.
- P2Y2 purinergic receptor gene deletion protects mice from bacterial endotoxin and sepsis-associated liver injury and mortality.American journal of physiology. Gastrointestinal and liver physiology · 2023Article
- Quinacrine is not a vital fluorescent probe for vesicular ATP storage.Purinergic signalling · 2021Article
- Lytic Release of Cellular ATP: Physiological Relevance and Therapeutic Applications.Life (Basel, Switzerland) · 2021Review
- Recent progress in developing fluorescent probes for imaging cell metabolites.Biomedical materials (Bristol, England) · 2021Review
- Airway Epithelial Nucleotide Release Contributes to Mucociliary Clearance.Life (Basel, Switzerland) · 2021Review
- Wide field of view quantitative imaging of cellular ATP release.American journal of physiology. Cell physiology · 2019Article
- Endoplasmic reticulum stress stimulates the release of extracellular vesicles carrying danger-associated molecular pattern (DAMP) molecules.Oncotarget · 2018Article
- Cell culture: complications due to mechanical release of ATP and activation of purinoceptors.Cell and tissue research · 2017Review
- Vesicular nucleotide transporter (VNUT): appearance of an actress on the stage of purinergic signaling.Purinergic signalling · 2017Review
- Cdon deficiency causes cardiac remodeling through hyperactivation of WNT/β-catenin signaling.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Imaging Adenosine Triphosphate (ATP).The Biological bulletin · 2016Review
- PKCα regulates TMEM16A-mediated Cl⁻ secretion in human biliary cells.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Vascular Calcification Revisited: A New Perspective for Phosphate TransportCurrent cardiology reviews · 2015Article
- Modular platform for low-light microscopy.Biomedical optics express · 2015Article
- Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.Nature communications · 2015Article
- Metabolic danger signals, uric acid and ATP, mediate inflammatory cross-talk between hepatocytes and immune cells in alcoholic liver disease.Journal of leukocyte biology · 2015Article
- Calcium signaling and secretion in cholangiocytes.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] · 2015Review
- Hypoxia is an effective stimulus for vesicular release of ATP from human umbilical vein endothelial cells.Placenta · 2015Article
- Cell death and DAMPs in acute pancreatitis.Molecular medicine (Cambridge, Mass.) · 2014Review
- Bacterial RTX toxins allow acute ATP release from human erythrocytes directly through the toxin pore.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Extracellular ATP represents an important autocrine/paracrine signaling molecule within the liver. The mechanisms responsible for ATP release are unknown, and alternative pathways have been proposed, including either conductive ATP movement through channels or exocytosis of ATP-enriched vesicles, although direct evidence from liver cells has been lacking. Utilizing dynamic imaging modalities (confocal and total internal reflection fluorescence microscopy and luminescence detection utilizing a high sensitivity CCD camera) at different scales, including confluent cell populations, single cells, and the intracellular submembrane space, we have demonstrated in a model liver cell line that (i) ATP release is not uniform but reflects point source release by a defined subset of cells; (ii) ATP within cells is localized to discrete zones of high intensity that are approximately 1 mum in diameter, suggesting a vesicular localization; (iii) these vesicles originate from a bafilomycin A(1)-sensitive pool, are depleted by hypotonic exposure, and are not rapidly replenished from recycling of endocytic vesicles; and (iv) exocytosis of vesicles in response to cell volume changes depends upon a complex series of signaling events that requires intact microtubules as well as phosphoinositide 3-kinase and protein kinase C. Collectively, these findings are most consistent with an essential role for exocytosis in regulated release of ATP and initiation of purinergic signaling in liver cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.