ArticleBMC cancer2010
Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues.
Article in BMC cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Establishment and characterization of three canine ameloblastoma cell lines with genomic and transcriptomic profiles.PloS one · 2026Article
- A network model for patient-derived drug response in breast cancer integrating multi-omics datasets.bioRxiv : the preprint server for biology · 2025Article
- Chromosomal Damage, Chromosome Instability, and Polymorphisms inInternational journal of molecular sciences · 2024Article
- Molecular Characterization and Landscape of Breast cancer Models from a multi-omics Perspective.Journal of mammary gland biology and neoplasia · 2023Review
- Computational estimation of quality and clinical relevance of cancer cell lines.Molecular systems biology · 2022Review
- Primary Lung Cancer Organoids for Personalized Medicine-Are They Ready for Clinical Use?Cancers · 2021Review
- A pan-cancer survey of cell line tumor similarity by feature-weighted molecular profiles.Cell reports methods · 2021Article
- Global computational alignment of tumor and cell line transcriptional profiles.Nature communications · 2021Article
- Three-DimensionalFrontiers in toxicology · 2021Review
- Organophosphate Pesticide Exposure and Breast Cancer Risk: A Rapid Review of Human, Animal, and Cell-Based Studies.International journal of environmental research and public health · 2020Review
- Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer.Journal of visualized experiments : JoVE · 2020Article
- CHD1L promotes cell cycle progression and cell motility by up-regulating MDM2 in breast cancer.American journal of translational research · 2019Article
- Establishment of two basal-like breast cancer cell lines with extremely low tumorigenicity from Taiwanese premenopausal women.Human cell · 2018Article
- Comparative genomic and genetic analysis of glioblastoma-derived brain tumor-initiating cells and their parent tumors.Neuro-oncology · 2016Article
- Article
- CoMAGC: a corpus with multi-faceted annotations of gene-cancer relations.BMC bioinformatics · 2013Article
- Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors.Oncology reports · 2013Article
- Patient-derived models of human breast cancer: protocols for in vitro and in vivo applications in tumor biology and translational medicine.Current protocols in pharmacology · 2013Article
- CHD1L Is a Marker for Poor Prognosis of Hepatocellular Carcinoma after Surgical Resection.Korean journal of pathology · 2013Article
- Development of a screen to identify selective small molecules active against patient-derived metastatic and chemoresistant breast cancer cells.Breast cancer research : BCR · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCell lines are commonly used in various kinds of biomedical research in the world. However, it remains uncertain whether genomic alterations existing in primary tumor tissues are represented in cell lines and whether cell lines carry cell line-specific genomic alterations. This study was performed to answer these questions.
methodsArray-based comparative genomic hybridization (CGH) was employed with 4030 bacterial artificial chromosomes (BACs) that cover the genome at 1.0 megabase resolution to analyze DNA copy number aberrations (DCNAs) in 35 primary breast tumors and 24 breast cancer cell lines. DCNAs were compared between these two groups. A tissue microdissection technique was applied to primary tumor tissues to reduce the contamination of samples by normal tissue components.
resultsThe average number of BAC clones with DCNAs was 1832 (45.3% of spotted clones) and 971 (24.9%) for cell lines and primary tumor tissues, respectively. Gains of 1q and 8q and losses of 8p, 11q, 16q and 17p were detected in >50% of primary cancer tissues. These aberrations were also frequently detected in cell lines. In addition to these alterations, the cell lines showed recurrent genomic alterations including gains of 5p14-15, 20q11 and 20q13 and losses of 4p13-p16, 18q12, 18q21, Xq21.1 and Xq26-q28 that were barely detected in tumor tissue specimens. These are considered to be cell line-specific DCNAs. The frequency of the HER2 amplification was high in both cell lines and tumor tissues, but it was statistically different between cell lines and primary tumors (P = 0.012); 41.3 +/- 29.9% for the cell lines and 15.9 +/- 18.6% for the tissue specimens.
conclusionsEstablished cell lines carry cell lines-specific DCNAs together with recurrent aberrations detected in primary tumor tissues. It must therefore be emphasized that cell lines do not always represent the genotypes of parental tumor tissues.
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