ArticleJournal of translational medicine2010
A quantitative real time PCR method to analyze T cell receptor Vbeta subgroup expansion by staphylococcal superantigens.
Article in Journal of translational medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
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- Blocking Superantigen-Mediated Diseases: Challenges and Future Trends.Journal of immunology research · 2024Review
- The superantigens SpeC and TSST-1 specifically activate TRBV12-3/12-4Communications biology · 2023Article
- T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignancies.Nature communications · 2022Article
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- Endogenous HLA-DQ8αβ programs superantigens (SEG/SEI) to silence toxicity and unleash a tumoricidal network with long-term melanoma survival.Journal for immunotherapy of cancer · 2020Article
- T Cell Immunity to Bacterial Pathogens: Mechanisms of Immune Control and Bacterial Evasion.International journal of molecular sciences · 2020Review
- Staphylococcus aureus Isolated from Skin from Atopic-Dermatitis Patients Produces Staphylococcal Enterotoxin Y, Which Predominantly Induces T-Cell Receptor Vα-Specific Expansion of T Cells.Infection and immunity · 2020Article
- Single-Cell Sequencing of T cell Receptors: A Perspective on the Technological Development and Translational Application.Advances in experimental medicine and biology · 2020Review
- Dendritic Cells InternalizeMicroorganisms · 2019Article
- The T cell activating properties and antitumour activity of Staphylococcal Enterotoxin-like Q.Medical microbiology and immunology · 2019Article
- Contact Dermatitis in Atopic Dermatitis Children-Past, Present, and Future.Clinical reviews in allergy & immunology · 2019Review
- Bovine Staphylococcus aureus Superantigens Stimulate the Entire T Cell Repertoire of Cattle.Infection and immunity · 2018Article
- The Role and Diagnosis of Allergic Contact Dermatitis in Patients with Atopic Dermatitis.American journal of clinical dermatology · 2018Review
- Manipulation of Innate and Adaptive Immunity by Staphylococcal Superantigens.Pathogens (Basel, Switzerland) · 2018Review
- Induction of Immunosuppressive CD8Journal of immunology (Baltimore, Md. : 1950) · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundStaphylococcal enterotoxins (SEs), SE-like (SEl) toxins, and toxic shock syndrome toxin-1 (TSST-1), produced by Staphylococcus aureus, belong to the subgroup of microbial superantigens (SAgs). SAgs induce clonal proliferation of T cells bearing specific variable regions of the T cell receptor beta chain (Vbeta). Quantitative real time PCR (qRT-PCR) has become widely accepted for rapid and reproducible mRNA quantification. Although the quantification of Vbeta subgroups using qRT-PCR has been reported, qRT-PCR using both primers annealing to selected Vbeta nucleotide sequences and SYBR Green I reporter has not been applied to assess Vbeta-dependent expansion of T cells by SAgs.
methodsHuman peripheral blood mononuclear cells were stimulated with various SAgs or a monoclonal antibody specific to human CD3. Highly specific expansion of Vbeta subgroups was assessed by qRT-PCR using SYBR Green I reporter and primers corresponding to selected Vbeta nucleotide sequences.
resultsqRT-PCR specificities were confirmed by sequencing amplified PCR products and melting curve analysis. To assess qRT-PCR efficiencies, standard curves were generated for each primer set. The average slope and R2 of standard curves were -3.3764 +/- 0.0245 and 0.99856 +/- 0.000478, respectively, demonstrating that the qRT-PCR established in this study is highly efficient. With some exceptions, SAg Vbeta specificities observed in this study were similar to those reported in previous studies.
conclusionsThe qRT-PCR method established in this study produced an accurate and reproducible assessment of Vbeta-dependent expansion of human T cells by staphylococcal SAgs. This method could be a useful tool in the characterization T cell proliferation by newly discovered SAg and in the investigation of biological effects of SAgs linked to pathogenesis.
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