ArticleGenome biology2010
Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts.
Article in Genome biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
104 citing papers in PubMed, 187 citations in OpenAlex.
- Actin from within - how nuclear myosins and actin regulate nuclear architecture and mechanics.Journal of cell science · 2025Review
- Interactions of Chromatin with the Nuclear Lamina and Nuclear Pore Complexes.International journal of molecular sciences · 2023Review
- Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters.Journal of extracellular biology · 2023Article
- Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa.International journal of biological sciences · 2023Article
- Review
- Chromatin Dynamics During Entry to Quiescence and Compromised Functionality in Cancer Cells.Results and problems in cell differentiation · 2022Article
- Nuclear organization and dynamics: The final Frontier for understanding genome regulation.Frontiers in cell and developmental biology · 2022Article
- Microgravity and space radiation inhibit autophagy in human capillary endothelial cells, through either opposite or synergistic effects on specific molecular pathways.Cellular and molecular life sciences : CMLS · 2021Article
- Nuclear deformation guides chromatin reorganization in cardiac development and disease.Nature biomedical engineering · 2021Article
- New Insights into Cellular Functions of Nuclear Actin.Biology · 2021Review
- Polymer modelling unveils the roles of heterochromatin and nucleolar organizing regions in shaping 3D genome organization in Arabidopsis thaliana.Nucleic acids research · 2021Article
- Rapid nucleus-scale reorganization of chromatin in neurons enables transcriptional adaptation for memory consolidation.PloS one · 2021Article
- Is There a Histone Code for Cellular Quiescence?Frontiers in cell and developmental biology · 2021Review
- Journey to the Center of the Cell: Cytoplasmic and Nuclear Actin in Immune Cell Functions.Frontiers in cell and developmental biology · 2021Review
- Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells.Frontiers in cell and developmental biology · 2021Article
- Inferring chromosome radial organization from Hi-C data.BMC bioinformatics · 2020Article
- Computational methods for predicting 3D genomic organization from high-resolution chromosome conformation capture data.Briefings in functional genomics · 2020Article
- Radial Organization in the Mammalian Nucleus.Frontiers in genetics · 2020Review
- Complex Chromatin Motions for DNA Repair.Frontiers in genetics · 2020Review
- Actin' between phase separated domains for heterochromatin repair.DNA repair · 2019Review
44 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRadial chromosome positioning in interphase nuclei is nonrandom and can alter according to developmental, differentiation, proliferation, or disease status. However, it is not yet clear when and how chromosome repositioning is elicited.
resultsBy investigating the positioning of all human chromosomes in primary fibroblasts that have left the proliferative cell cycle, we have demonstrated that in cells made quiescent by reversible growth arrest, chromosome positioning is altered considerably. We found that with the removal of serum from the culture medium, chromosome repositioning took less than 15 minutes, required energy and was inhibited by drugs affecting the polymerization of myosin and actin. We also observed that when cells became quiescent, the nuclear distribution of nuclear myosin 1 beta was dramatically different from that in proliferating cells. If we suppressed the expression of nuclear myosin 1 beta by using RNA-interference procedures, the movement of chromosomes after 15 minutes in low serum was inhibited. When high serum was restored to the serum-starved cultures, chromosome repositioning was evident only after 24 to 36 hours, and this coincided with a return to a proliferating distribution of nuclear myosin 1 beta.
conclusionsThese findings demonstrate that genome organization in interphase nuclei is altered considerably when cells leave the proliferative cell cycle and that repositioning of chromosomes relies on efficient functioning of an active nuclear motor complex that contains nuclear myosin 1 beta.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.