ArticleJournal of neurochemistry2010
Both synthesis and reuptake are critical for replenishing the releasable serotonin pool in Drosophila.
Article in Journal of neurochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- Tetrodotoxin Oral Film Attenuates Depression in a Chronic Unpredictable Mild Stress Model in Mice.Marine drugs · 2026Article
- Microdosing ketamine in Drosophila does not block serotonin reuptake, but causes complex behavioral changes mediated by glutamate and serotonin receptors.Journal of neurochemistry · 2024Article
- Electrochemical and biosensor techniques to monitor neurotransmitter changes with depression.Analytical and bioanalytical chemistry · 2024Review
- Microdosing ketamine inbioRxiv : the preprint server for biology · 2023Article
- Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science.Current protocols · 2023Review
- Regulation and modulation of biogenic amine neurotransmission inFrontiers in physiology · 2023Review
- SSRI antidepressants differentially modulate serotonin reuptake and release in Drosophila.Journal of neurochemistry · 2022Article
- TheGenome research · 2021Article
- Improving serotonin fast-scan cyclic voltammetry detection: new waveforms to reduce electrode fouling.The Analyst · 2020Article
- Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging.iScience · 2019Article
- Drosophila as a Model System for Neurotransmitter Measurements.ACS chemical neuroscience · 2018Review
- Fast-Scan Cyclic Voltammetry (FSCV) Detection of Endogenous Octopamine in Drosophila melanogaster Ventral Nerve Cord.ACS chemical neuroscience · 2016Article
- Comparison of dopamine kinetics in the larval Drosophila ventral nerve cord and protocerebrum with improved optogenetic stimulation.Journal of neurochemistry · 2015Article
- Characterization of dopamine releasable and reserve pools in Drosophila larvae using ATP/P2X2 -mediated stimulation.Journal of neurochemistry · 2015Article
- Analysis of neurotransmitter tissue content of Drosophila melanogaster in different life stages.ACS chemical neuroscience · 2015Article
- Optogenetic control of serotonin and dopamine release in Drosophila larvae.ACS chemical neuroscience · 2014Article
- Drosophila melanogaster as a genetic model system to study neurotransmitter transporters.Neurochemistry international · 2014Review
- A comparison of the subsecond dynamics of neurotransmission of dopamine and serotonin.ACS chemical neuroscience · 2013Review
- Kinetics of the dopamine transporter in Drosophila larva.ACS chemical neuroscience · 2013Article
- Freeze-drying as sample preparation for micellar electrokinetic capillary chromatography-electrochemical separations of neurochemicals in Drosophila brains.Analytical chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The two main sources of serotonin available for release are expected to be newly synthesized serotonin and serotonin recycled after reuptake by the serotonin transporter. However, their relative importance for maintaining release and the time course of regulation are unknown. We studied serotonin signaling in the ventral nerve cord of the larval Drosophila CNS. Fast-scan cyclic voltammetry at implanted microelectrodes was used to detect serotonin elicited by channelrhodopsin2-mediated depolarization. The effects of reuptake were probed by incubating in cocaine, which is selective for the serotonin transporter in Drosophila. p-chlorophenylalanine, an inhibitor of tryptophan hydroxylase2, was used to investigate the effects of synthesis. Stimulations were repeated at various intervals to assess the time course of recovery of the releasable pool. Reuptake is important for the rapid replenishment of the releasable pool, on the 1 min time scale. Synthesis is critical to the longer-term replenishment (10 min) of the releasable pool, especially when reuptake is also inhibited. Concurrent synthesis and reuptake inhibition decreased both serotonin tissue content measured by immunohistochemistry (by 50%) and the initial amount of evoked serotonin (by 65%). Decreases in evoked serotonin are rescued by inhibiting action potential propagation with tetrodotoxin, implicating endogenous activity in the depletion. These results show synthesis is necessary to replenish part of the releasable serotonin pool that is depleted after reuptake inhibition, suggesting that regulation of synthesis may modulate the effects of serotonin reuptake inhibitors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.