ArticleCellular microbiology2010
Cryptococcus neoformans responds to mannitol by increasing capsule size in vitro and in vivo.
Article in Cellular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 58 citations in OpenAlex.
- The Cryptococcus neoformans titanide is a pathogenic cell type that arises from typical yeast cells in response to host conditions.PLoS biology · 2026Article
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- Ubiquitin degradation of the Ribonuclease H2 subunit B Rnh2B regulates the virulence ofVirulence · 2025Article
- Pathogenicity and virulence of Cryptococcus neoformans from an environmental perspective.Virulence · 2025Review
- Vacuolar protein sorting-associated protein Vps17 regulates sexual reproduction and virulence ofMycology · 2025Article
- Research advances and public health strategies in China on WHO priority fungal pathogens.Mycology · 2025Review
- Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction ofJournal of fungi (Basel, Switzerland) · 2023Article
- Recognition of Cell Wall Mannosylated Components as a Conserved Feature for Fungal Entrance, Adaptation and Survival Within Trophozoites ofFrontiers in cellular and infection microbiology · 2022Article
- Influence of Pathogen Carbon Metabolism on Interactions With Host Immunity.Frontiers in cellular and infection microbiology · 2022Review
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- The F-Box Protein Fbp1 Regulates Virulence ofFrontiers in cellular and infection microbiology · 2021Article
- Protective Efficacy of Lectin-Fc(IgG) Fusion Proteins In Vitro and in a Pulmonary Aspergillosis In Vivo Model.Journal of fungi (Basel, Switzerland) · 2020Article
- Article
- The capsule ofVirulence · 2019Review
- The 'Amoeboid Predator-Fungal Animal Virulence' Hypothesis.Journal of fungi (Basel, Switzerland) · 2019Review
- Unraveling synthesis of the cryptococcal cell wall and capsule.Glycobiology · 2018Review
- Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body.Journal of visualized experiments : JoVE · 2018Article
- Nutritional Requirements and Their Importance for Virulence of Pathogenic Cryptococcus Species.Microorganisms · 2017Review
- Characterization of the antifungal functions of a WGA-Fc (IgG2a) fusion protein binding to cell wall chitin oligomers.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The polysaccharide capsule of the fungus Cryptococcus neoformans is its main virulence factor. In this study, we determined the effects of mannitol and glucose on the capsule and exopolysaccharide production. Growth in mannitol significantly increased capsular volume compared with cultivation in glucose. However, cells grown in glucose concentrations higher than 62.5 mM produced more exopolysaccharide than cells grown in mannitol. The fibre lengths and glycosyl composition of capsular polysaccharide from yeast grown in mannitol was structurally different from that of yeast grown in glucose. Furthermore, mannitol treatment of mice infected intratracheally with C. neoformans resulted in fungal cells with significantly larger capsules and the mice had reduced fungal dissemination to the brain. Our results demonstrate the capacity of carbohydrate source and concentration to modify the expression of a major virulence factor of C. neoformans. These findings may impact the clinical management of cryptococcosis.
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Registered trials
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