ReviewThe Biochemical journal2010
Taking the plunge: integrating structural, enzymatic and computational insights into a unified model for membrane-immersed rhomboid proteolysis.
Review in The Biochemical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 64 citations in OpenAlex.
- Phosphatidylglyerol Lipid Binding at the Active Site of an Intramembrane Protease.The Journal of membrane biology · 2020Review
- How does the exosite of rhomboid protease affect substrate processing and inhibition?Protein science : a publication of the Protein Society · 2017Article
- The Rhomboid Protease GlpG Promotes the Persistence of Extraintestinal Pathogenic Escherichia coli within the Gut.Infection and immunity · 2017Article
- A Golgi rhomboid protease Rbd2 recruits Cdc48 to cleave yeast SREBP.The EMBO journal · 2016Article
- Reversible Unfolding of Rhomboid Intramembrane Proteases.Biophysical journal · 2016Article
- Binding of longer Aβ to transmembrane domain 1 of presenilin 1 impacts on Aβ42 generation.Molecular neurodegeneration · 2014Article
- Proteolysis inside the membrane is a rate-governed reaction not driven by substrate affinity.Cell · 2013Article
- Novel tripod amphiphiles for membrane protein analysis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2013Article
- Residues in conserved loops of intramembrane metalloprotease SpoIVFB interact with residues near the cleavage site in pro-σK.Journal of bacteriology · 2013Article
- Topology of the yeast Ras converting enzyme as inferred from cysteine accessibility studies.Biochemistry · 2013Article
- The mammalian endoplasmic reticulum-associated degradation system.Cold Spring Harbor perspectives in biology · 2013Review
- Features of Pro-σK important for cleavage by SpoIVFB, an intramembrane metalloprotease.Journal of bacteriology · 2013Article
- Structure of the integral membrane protein CAAX protease Ste24p.Science (New York, N.Y.) · 2013Article
- Glucose-neopentyl glycol (GNG) amphiphiles for membrane protein study.Chemical communications (Cambridge, England) · 2013Article
- Activity-based probes for rhomboid proteases discovered in a mass spectrometry-based assay.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Making the cut: intramembrane cleavage by a rhomboid protease promotes ERAD.Nature structural & molecular biology · 2012Article
- Architectural and thermodynamic principles underlying intramembrane protease function.Nature chemical biology · 2012Article
- A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Article
- An internal water-retention site in the rhomboid intramembrane protease GlpG ensures catalytic efficiency.Structure (London, England : 1993) · 2012Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Rhomboid proteases are a fascinating class of enzymes that combine a serine protease active site within the core of an integral membrane protein. Despite having key roles in animal cell signalling and microbial pathogenesis, the membrane-immersed nature of these enzymes had long imposed obstacles to elucidating their biochemical mechanisms. But recent multidisciplinary approaches, including eight crystal structures, four computer simulations and nearly 100 engineered mutants interrogated in vivo and in vitro, are coalescing into an integrated model for one rhomboid orthologue in particular, bacterial GlpG. The protein creates a central hydrated microenvironment immersed below the membrane surface to support hydrolysis by its serine protease-like catalytic apparatus. Four conserved architectural elements in particular act as 'keystones' to stabilize this structure, and the lateral membrane-embedded L1 loop functions as a 'flotation device' to position the protease tilted in the membrane. Complex interplay between lateral substrate gating by rhomboid, substrate unwinding and local membrane thinning leads to intramembrane proteolysis of selected target proteins. Although far from complete, studies with GlpG currently offer the best prospect for achieving a thorough and sophisticated understanding of a simplified intramembrane protease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.