ArticleBiotechnology and bioengineering2010
Three-dimensional cell culture microarray for high-throughput studies of stem cell fate.
Article in Biotechnology and bioengineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 108 citations in OpenAlex.
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Application and prospects of high-throughput screening forWorld journal of stem cells · 2022Review
- The impact of microfluidics in high-throughput drug-screening applications.Biomicrofluidics · 2022Review
- Fabrication approaches for high-throughput and biomimetic disease modeling.Acta biomaterialia · 2021Review
- Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis.World journal of stem cells · 2019Review
- Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays.Journal of visualized experiments : JoVE · 2017Article
- Spatially and Temporally Controlled Hydrogels for Tissue Engineering.Materials science & engineering. R, Reports : a review journal · 2017Article
- Engineering complex tissue-like microgel arrays for evaluating stem cell differentiation.Scientific reports · 2016Article
- Living Cell Microarrays: An Overview of Concepts.Microarrays (Basel, Switzerland) · 2016Review
- Hydrogel-laden paper scaffold system for origami-based tissue engineering.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Review
- Review
- Three-dimensional, soft neotissue arrays as high throughput platforms for the interrogation of engineered tissue environments.Biomaterials · 2015Article
- Sample preparation strategies for mass spectrometry imaging of 3D cell culture models.Journal of visualized experiments : JoVE · 2014Article
- New Methods in Tissue Engineering: Improved Models for Viral Infection.Annual review of virology · 2014Article
- Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages.Biochimica et biophysica acta · 2014Article
- Fibroblast growth factor-based signaling through synthetic heparan sulfate blocks copolymers studied using high cell density three-dimensional cell printing.The Journal of biological chemistry · 2014Article
- Microarray platform affords improved product analysis in mammalian cell growth studies.Biotechnology journal · 2014Article
- A combinatorial cell-laden gel microarray for inducing osteogenic differentiation of human mesenchymal stem cells.Scientific reports · 2014Article
- Development of functional biomaterials with micro- and nanoscale technologies for tissue engineering and drug delivery applications.Journal of tissue engineering and regenerative medicine · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
We have developed a novel three-dimensional (3D) cellular microarray platform to enable the rapid and efficient tracking of stem cell fate and quantification of specific stem cell markers. This platform consists of a miniaturized 3D cell culture array on a functionalized glass slide for spatially addressable high-throughput screening. A microarray spotter was used to deposit cells onto a modified glass surface to yield an array consisting of cells encapsulated in alginate gel spots with volumes as low as 60 nL. A method based on an immunofluorescence technique scaled down to function on a cellular microarray was also used to quantify specific cell marker protein levels in situ. Our results revealed that this platform is suitable for studying the expansion of mouse embryonic stem (ES) cells as they retain their pluripotent and undifferentiated state. We also examined neural commitment of mouse ES cells on the microarray and observed the generation of neuroectodermal precursor cells characterized by expression of the neural marker Sox-1, whose levels were also measured in situ using a GFP reporter system. In addition, the high-throughput capacity of the platform was tested using a dual-slide system that allowed rapid screening of the effects of tretinoin and fibroblast growth factor-4 (FGF-4) on the pluripotency of mouse ES cells. This high-throughput platform is a powerful new tool for investigating cellular mechanisms involved in stem cell expansion and differentiation and provides the basis for rapid identification of signals and conditions that can be used to direct cellular responses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.