ArticleCurrent protocols in cytometry2010
Use of flow cytometric methods to quantify protein-protein interactions.
Article in Current protocols in cytometry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- A multiplex extracellular interactome screening method employing high-avidity nanoparticles.bioRxiv : the preprint server for biology · 2025Article
- Comprehensive normalization and binary classification methods for enhanced sensitivity and reproducibility in Luminex assay quantitation.Journal of immunological methods · 2025Article
- Dectin-1 stimulating β-glucans inhibit Chlamydia infections both in vitro and in vivo.Pathogens and disease · 2025Article
- Quantification of Binding of Small Molecules to Native Proteins Overexpressed in Living Cells.Journal of the American Chemical Society · 2024Article
- Simultaneous blocking of the pan-RAF and S100B pathways as a synergistic therapeutic strategy against malignant melanoma.Journal of cellular and molecular medicine · 2021Article
- An Interhelical Salt Bridge Controls Flexibility and Inhibitor Potency for Regulators of G-protein Signaling Proteins 4, 8, and 19.Molecular pharmacology · 2019Article
- Interplay of cysteine exposure and global protein dynamics in small-molecule recognition by a regulator of G-protein signaling protein.Proteins · 2019Article
- Principles of Inter-Amino-Acid Recognition Revealed by Binding Energies between Homogeneous Oligopeptides.ACS central science · 2019Article
- Dissecting the telomere-inner nuclear membrane interface formed in meiosis.Nature structural & molecular biology · 2017Article
- Article
- Flow Cytometry: Impact on Early Drug Discovery.Journal of biomolecular screening · 2015Review
- An interdomain boundary in RAG1 facilitates cooperative binding to RAG2 in formation of the V(D)J recombinase complex.Protein science : a publication of the Protein Society · 2015Article
- Improvement of the fluorescence intensity during a flow cytometric analysis for rice protoplasts by localization of a green fluorescent protein into chloroplasts.International journal of molecular sciences · 2014Article
- Hsp70-Bag3 interactions regulate cancer-related signaling networks.Cancer research · 2014Article
- Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro.The Journal of biological chemistry · 2014Article
- Conformational dynamics of a regulator of G-protein signaling protein reveals a mechanism of allosteric inhibition by a small molecule.ACS chemical biology · 2013Article
- Reversible inhibitors of regulators of G-protein signaling identified in a high-throughput cell-based calcium signaling assay.Cellular signalling · 2013Article
- Development of a capillary electrophoresis platform for identifying inhibitors of protein-protein interactions.Analytical chemistry · 2013Article
- Broad ranges of affinity and specificity of anti-histone antibodies revealed by a quantitative peptide immunoprecipitation assay.Journal of molecular biology · 2012Article
- Molecular mechanism for inhibition of g protein-coupled receptor kinase 2 by a selective RNA aptamer.Structure (London, England : 1993) · 2012Article
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4 authors.
Funding
Abstract
A method is described for the quantitative analysis of protein-protein interactions using the flow cytometry protein interaction assay (FCPIA). This method is based upon immobilizing protein on a polystyrene bead, incubating these beads with a fluorescently labeled binding partner, and assessing the sample for bead-associated fluorescence in a flow cytometer. This method can be used to calculate protein-protein interaction affinities or to perform competition experiments with unlabeled binding partners or small molecules. Examples described in this protocol highlight the use of this assay in the quantification of the affinity of binding partners of the regulator of G-protein signaling protein, RGS19, in either a saturation or a competition format. An adaptation of this method that is compatible for high-throughput screening is also provided.
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