ArticleJournal of cell science2010
Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster.
Article in Journal of cell science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- ARF GTPases and Their Ubiquitous Role in Intracellular Trafficking Beyond the Golgi.Frontiers in cell and developmental biology · 2021Review
- Induction of membrane curvature by proteins involved in Golgi trafficking.Advances in biological regulation · 2020Review
- The roles of Syx5 in Golgi morphology and Rhodopsin transport in Drosophila photoreceptors.Biology open · 2016Article
- Rab6 Is Required for Multiple Apical Transport Pathways but Not the Basolateral Transport Pathway in Drosophila Photoreceptors.PLoS genetics · 2016Article
- Vectors for Genetically-Encoded Tags for Electron Microscopy Contrast in Drosophila.Biological procedures online · 2016Article
- The lysosomal enzyme receptor protein (LERP) is not essential, but is implicated in lysosomal function in Drosophila melanogaster.Biology open · 2015Article
- The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo.Nature communications · 2015Article
- Transcriptional regulation of secretory capacity by bZip transcription factors.Frontiers in biology · 2015Article
- Drosophila melanogaster cellular repressor of E1A-stimulated genes is a lysosomal protein essential for fly development.Biochimica et biophysica acta · 2014Article
- Trans-Golgi network-located AP1 gamma adaptins mediate dileucine motif-directed vacuolar targeting in Arabidopsis.The Plant cell · 2014Article
- Drosophila rugose is a functional homolog of mammalian Neurobeachin and affects synaptic architecture, brain morphology, and associative learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Type II phosphatidylinositol 4-kinase regulates trafficking of secretory granule proteins in Drosophila.Development (Cambridge, England) · 2012Article
- Article
- Drosophila GGA model: an ultimate gateway to GGA analysis.Traffic (Copenhagen, Denmark) · 2011Article
- Regulation of β2-adrenergic receptor maturation and anterograde trafficking by an interaction with Rab geranylgeranyltransferase: modulation of Rab geranylgeranylation by the receptor.The Journal of biological chemistry · 2011Article
- GCC185 plays independent roles in Golgi structure maintenance and AP-1-mediated vesicle tethering.The Journal of cell biology · 2011Article
- AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila.Molecular biology of the cell · 2011Article
- A potential role for the clathrin adaptor GGA in Drosophila spermatogenesis.BMC cell biology · 2011Article
- Diversity in protein glycosylation among insect species.PloS one · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Targeting of proteins to their final destination is a prerequisite for living cells to maintain their homeostasis. Clathrin functions as a coat that forms transport carriers called clathrin-coated vesicles (CCVs) at the plasma membrane and post-Golgi compartments. In this study, we established an experimental system using Schneider S2 cells derived from the fruit fly, Drosophila melanogaster, as a model system to study the physiological roles of clathrin adaptors, and to dissect the processes of CCV formation. We found that a clathrin adaptor Drosophila GGA (dGGA), a homolog of mammalian GGA proteins, localizes to the trans-Golgi network (TGN) and is capable of recruiting clathrin from the cytosol onto TGN membranes. dGGA itself is recruited from the cytosol to the TGN in an ARF1 small GTPase (dARF79F)-dependent manner. dGGA recognizes the cytoplasmic acidic-cluster-dileucine (ACLL) sorting signal of Lerp (lysosomal enzyme receptor protein), a homolog of mammalian mannose 6-phosphate receptors. Moreover, both dGGA and another type of TGN-localized clathrin adaptor, AP-1 (adaptor protein-1 complex), are shown to be involved in the trafficking of Lerp from the TGN to endosomes and/or lysosomes. Taken together, our findings indicate that the protein-sorting machinery in fly cells is well conserved relative to that in mammals, enabling the use of fly cells to dissect CCV biogenesis and clathrin-dependent protein trafficking at the TGN of higher eukaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.