ArticleExperimental cell research2010
Mesenchymal cells stimulate capillary morphogenesis via distinct proteolytic mechanisms.
Article in Experimental cell research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed, 156 citations in OpenAlex.
- Harnessing inter-spheroid spacing and structural connectivity to direct collective cell migration and host vessel integration in 3D engineered tissue.Materials today. Bio · 2026Article
- Active Microrheology Reveals Distinct ECM Mechanical Signatures Induced by Stromal Cells of Different Tissue Origins during Vascular Morphogenesis.ACS biomaterials science & engineering · 2026Article
- Zinc-Releasing Fibrous Scaffolds Modulate Fibroblast, Endothelial, and Macrophage Interactions for Vascularized Tissue Engineering.ACS applied materials & interfaces · 2026Article
- Free-floating long-term vascularized mesenchymal organoids.iScience · 2026Article
- Dynamic microenvironments for bone organoids: spatiotemporal regulation of angiogenic-osteogenic coupling through advanced hydrogel design.Frontiers in bioengineering and biotechnology · 2026Review
- Forskolin Enhances Urokinase Plasminogen Activator Secretion and Angiogenic Activity of Xeno-Free Cultures of Human Adipose Tissue-Derived Stem Cells.Advanced biology · 2025Article
- Tranexamic acid impairs plasmin generation on human mesenchymal stem cells and derived membrane microvesicles, halting pericellular proteolysis.Frontiers in medicine · 2025Article
- In vitro biomaterial priming of human mesenchymal stromal/stem cells : implication of the Src/JAK/STAT3 pathway in vasculogenic mimicry.Scientific reports · 2024Article
- Engineering primitive multiscale chimeric vasculature by combining human microvessels with explanted murine vessels.Scientific reports · 2024Article
- Manufacturing the multiscale vascular hierarchy: progress toward solving the grand challenge of tissue engineering.Trends in biotechnology · 2023Review
- Neuro-regenerative behavior of adipose-derived stem cells in aligned collagen I hydrogels.Materials today. Bio · 2023Article
- Engineering Cell Microenvironment Using Nanopattern-Derived Multicellular Spheroids and Photo-Crosslinked Gelatin/Hyaluronan Hydrogels.Polymers · 2023Article
- 12-Oxoeicosatetraenoic acid, a candidate signal for placenta separation, activates matrix metalloproteinase and induces apoptosis in bovine trophoblast cells.Animal bioscience · 2023Article
- Mesenchymal stromal cell spheroids in sulfated alginate enhance muscle regeneration.Acta biomaterialia · 2023Article
- Tumorigenic effects of human mesenchymal stromal cells and fibroblasts on bladder cancer cells.Frontiers in oncology · 2023Article
- Identification of molecularly unique tumor-associated mesenchymal stromal cells in breast cancer patients.PloS one · 2023Article
- Organotypic stromal cells impact endothelial cell transcriptome in 3D microvessel networks.Scientific reports · 2022Article
- A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis.Bioengineering (Basel, Switzerland) · 2022Article
- Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line.BMC molecular and cell biology · 2022Article
- Phenotypic, trophic, and regenerative properties of mesenchymal stem cells from different osseous tissues.Cell and tissue research · 2022Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
During angiogenesis, endothelial cells (ECs) degrade their surrounding extracellular matrix (ECM) to facilitate invasion. How interactions between ECs and other cells within their microenvironment facilitate this process is only partially understood. We have utilized a tractable 3D co-culture model to investigate the proteolytic mechanisms by which pre-committed or more highly committed mesenchymal cells stimulate capillary formation. On their own, ECs invade their surrounding matrix, but do not form capillaries. However, in the presence of either mesenchymal stem cells (MSCs) or fibroblasts, ECs form polarized, tubular structures that are intimately associated with mesenchymal cells. Further, ECs up-regulate gene expression of several extracellular proteases upon co-culture with either mesenchymal cell type. The administration of both broad spectrum and specific protease inhibitors demonstrated that MSC-stimulated capillary formation relied solely on membrane-type matrix metalloproteinases (MT-MMPs) while fibroblast-mediated sprouting proceeded independent of MMP inhibition unless the plasminogen activator/plasmin axis was inhibited in concert. While other studies have established a role for the ECM itself in dictating proteolysis and matrix degradation during capillary morphogenesis, the present study illustrates that heterotypic cellular interactions within the microenvironment can direct the proteolytic mechanisms required for capillary formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.