ArticleBMC microbiology2010
New structural and functional defects in polyphosphate deficient bacteria: a cellular and proteomic study.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- The roles and synthesis of inorganic polyphosphate inbioRxiv : the preprint server for biology · 2026Article
- Article
- Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology inbioRxiv : the preprint server for biology · 2024Article
- Inorganic phosphate modifies stationary phase fitness and metabolic pathways inFrontiers in microbiology · 2024Article
- The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation inMicroorganisms · 2021Article
- Biogeochemical Niche of Magnetotactic Cocci Capable of Sequestering Large Polyphosphate Inclusions in the Anoxic Layer of the Lake Pavin Water Column.Frontiers in microbiology · 2021Article
- Transcriptional Responses ofFrontiers in microbiology · 2021Article
- LeveragingFrontiers in microbiology · 2021Review
- Sulfurimonas subgroup GD17 cells accumulate polyphosphate under fluctuating redox conditions in the Baltic Sea: possible implications for their ecology.The ISME journal · 2019Article
- Fluorescence enzymatic assay for bacterial polyphosphate kinase 1 (PPK1) as a platform for screening antivirulence molecules.Infection and drug resistance · 2019Article
- Inorganic Polyphosphate Is Essential forFrontiers in cellular and infection microbiology · 2018Article
- Datasets for transcriptomics, q-proteomics and phenotype microarrays of polyphosphate metabolism mutants fromData in brief · 2017Article
- Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Polyphosphate: popping up from oblivion.Current genetics · 2017Review
- Proteomics dedicated to biofilmology: What have we learned from a decade of research?Medical microbiology and immunology · 2016Review
- Human serum transferrin fibrils: nanomineralisation in bacteria and destruction of red blood cells.Chembiochem : a European journal of chemical biology · 2015Article
- Transcriptome analysis of Campylobacter jejuni polyphosphate kinase (ppk1 and ppk2) mutants.Virulence · 2015Article
- Botrytis cinerea protein O-mannosyltransferases play critical roles in morphogenesis, growth, and virulence.PloS one · 2013Article
- Article
- Inorganic polyphosphates in extremophiles and their possible functions.Extremophiles : life under extreme conditions · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInorganic polyphosphate (polyP), a polymer of tens or hundreds of phosphate residues linked by ATP-like bonds, is found in all organisms and performs a wide variety of functions. PolyP is synthesized in bacterial cells by the actions of polyphosphate kinases (PPK1 and PPK2) and degraded by exopolyphosphatase (PPX). Bacterial cells with polyP deficiencies due to knocking out the ppk1 gene are affected in many structural and important cellular functions such as motility, quorum sensing, biofilm formation and virulence among others. The cause of this pleiotropy is not entirely understood.
resultsThe overexpression of exopolyphosphatase in bacteria mimicked some pleitropic defects found in ppk1 mutants. By using this approach we found new structural and functional defects in the polyP-accumulating bacteria Pseudomonas sp. B4, which are most likely due to differences in the polyP-removal strategy. Colony morphology phenotype, lipopolysaccharide (LPS) structure changes and cellular division malfunction were observed. Finally, we used comparative proteomics in order to elucidate the cellular adjustments that occurred during polyP deficiency in this bacterium and found some clues that helped to understand the structural and functional defects observed.
conclusionsThe results obtained suggest that during polyP deficiency energy metabolism and particularly nucleoside triphosphate (NTP) formation were affected and that bacterial cells overcame this problem by increasing the flux of energy-generating metabolic pathways such as tricarboxilic acid (TCA) cycle, beta-oxidation and oxidative phosphorylation and by reducing energy-consuming ones such as active transporters and amino acid biosynthesis. Furthermore, our results suggest that a general stress response also took place in the cell during polyP deficiency.
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Registered trials
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