ArticleAnalytical chemistry2010
Incorporation of the fluorescent ribonucleotide analogue tCTP by T7 RNA polymerase.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
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- Live-Cell RNA Imaging with Metabolically Incorporated Fluorescent Nucleosides.Journal of the American Chemical Society · 2022Article
- Fluorescent base analogues in gapmers enable stealth labeling of antisense oligonucleotide therapeutics.Scientific reports · 2021Article
- Stealth Fluorescence Labeling for Live Microscopy Imaging of mRNA Delivery.Journal of the American Chemical Society · 2021Article
- Compatibility and Fidelity of Mirror-Image Thymidine in Transcription Events by T7 RNA Polymerase.Molecular therapy. Nucleic acids · 2020Article
- Profiling Cytidine Acetylation with Specific Affinity and Reactivity.ACS chemical biology · 2017Article
- Synthesis and polymerase activity of a fluorescent cytidine TNA triphosphate analogue.Nucleic acids research · 2017Article
- Fluorescent RNA cytosine analogue - an internal probe for detailed structure and dynamics investigations.Scientific reports · 2017Article
- Fluorescence Turn-On Sensing of DNA Duplex Formation by a Tricyclic Cytidine Analogue.Journal of the American Chemical Society · 2017Article
- Synthesis and evaluation of an alkyne-modified ATP analog for enzymatic incorporation into RNA.Bioorganic & medicinal chemistry letters · 2016Article
- Isomorphic emissive GTP surrogate facilitates initiation and elongation of in vitro transcription reactions.Journal of the American Chemical Society · 2014Article
- Functionalized tricyclic cytosine analogues provide nucleoside fluorophores with improved photophysical properties and a range of solvent sensitivities.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- Enzymatic incorporation of an azide-modified UTP analog into oligoribonucleotides for post-transcriptional chemical functionalization.Nature protocols · 2012Article
- Novel modifications in RNA.ACS chemical biology · 2012Review
- Effect of transition metal ions on the fluorescence and Taq-catalyzed polymerase chain reaction of tricyclic cytidine analogs.Analytical biochemistry · 2011Article
- Assembly of multifunctional phi29 pRNA nanoparticles for specific delivery of siRNA and other therapeutics to targeted cells.Methods (San Diego, Calif.) · 2011Review
- A boost for the emerging field of RNA nanotechnology.ACS nano · 2011Article
- Characterization of photophysical and base-mimicking properties of a novel fluorescent adenine analogue in DNA.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescent RNA is an important analytical tool in medical diagnostics, RNA cytochemistry, and RNA aptamer development. We have synthesized the fluorescent ribonucleotide analogue 1,3-diaza-2-oxophenothiazine-ribose-5'-triphosphate (tCTP) and tested it as substrate for T7 RNA polymerase in transcription reactions, a convenient route for generating RNA in vitro. When transcribing a guanine, T7 RNA polymerase incorporates tCTP with 2-fold higher catalytic efficiency than CTP and efficiently polymerizes additional NTPs onto the tC. Remarkably, T7 RNA polymerase does not incorporate tCTP with the same ambivalence opposite guanine and adenine with which DNA polymerases incorporate the analogous dtCTP. While several DNA polymerases discriminated against a d(tC-A) base pair only by factors <10, T7 RNA polymerase discriminates against tC-A base pair formation by factors of 40 and 300 when operating in the elongation and initiation mode, respectively. These catalytic properties make T7 RNA polymerase an ideal tool for synthesizing large fluorescent RNA, as we demonstrated by generating a approximately 800 nucleotide RNA in which every cytosine was replaced with tC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.