ArticleThe Journal of cell biology2010
Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 107 citations in OpenAlex.
- The power of many: when genetics met yeasts and high-throughput.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Multifaceted roles of SUMO in DNA metabolism.Nucleus (Austin, Tex.) · 2024Review
- Application of unimodal probability distribution models for morphological phenotyping of budding yeast.FEMS yeast research · 2024Article
- Origins, evolution, and physiological implications of de novo genes in yeast.Yeast (Chichester, England) · 2022Review
- Powering morphogenesis: multiscale challenges at the interface of cell adhesion and the cytoskeleton.Molecular biology of the cell · 2022Review
- Phenomics approaches to understand genetic networks and gene function in yeast.Biochemical Society transactions · 2022Article
- Single-cell image analysis to explore cell-to-cell heterogeneity in isogenic populations.Cell systems · 2021Review
- Systematic High-Content Screening of Fluorescently Tagged Yeast Double Mutant Strains.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Genetic interactions derived from high-throughput phenotyping of 6589 yeast cell cycle mutants.NPJ systems biology and applications · 2020Article
- From observing to predicting single-cell structure and function with high-throughput/high-content microscopy.Essays in biochemistry · 2019Review
- High-throughput time-resolved morphology screening in bacteria reveals phenotypic responses to antibiotics.Communications biology · 2019Article
- Widespread Cumulative Influence of Small Effect Size Mutations on Yeast Quantitative Traits.Cell systems · 2018Article
- Candida albicans Cdc15 is essential for mitotic exit and cytokinesis.Scientific reports · 2018Article
- Identification of functional modules induced by bare-metal stents and paclitaxel-eluting stents in coronary heart disease.Experimental and therapeutic medicine · 2018Article
- Systematic Gene-to-Phenotype Arrays: A High-Throughput Technique for Molecular Phenotyping.Molecular cell · 2018Article
- Fast-Growing Engineered Microbes: New Concerns for Gain-of-Function Research?Frontiers in genetics · 2018Review
- She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain.Nature communications · 2017Article
- Accurate Classification of Protein Subcellular Localization from High-Throughput Microscopy Images Using Deep Learning.G3 (Bethesda, Md.) · 2017Article
- Functional Analysis of Kinases and Transcription Factors inG3 (Bethesda, Md.) · 2017Article
- Exploring Quantitative Yeast Phenomics with Single-Cell Analysis of DNA Damage Foci.Cell systems · 2016Article
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Authors and funding
16 authors at 5 institutions in 3 countries.
Funding
Abstract
We describe the application of a novel screening approach that combines automated yeast genetics, synthetic genetic array (SGA) analysis, and a high-content screening (HCS) system to examine mitotic spindle morphogenesis. We measured numerous spindle and cellular morphological parameters in thousands of single mutants and corresponding sensitized double mutants lacking genes known to be involved in spindle function. We focused on a subset of genes that appear to define a highly conserved mitotic spindle disassembly pathway, which is known to involve Ipl1p, the yeast aurora B kinase, as well as the cell cycle regulatory networks mitotic exit network (MEN) and fourteen early anaphase release (FEAR). We also dissected the function of the kinetochore protein Mcm21p, showing that sumoylation of Mcm21p regulates the enrichment of Ipl1p and other chromosomal passenger proteins to the spindle midzone to mediate spindle disassembly. Although we focused on spindle disassembly in a proof-of-principle study, our integrated HCS-SGA method can be applied to virtually any pathway, making it a powerful means for identifying specific cellular functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.