ArticleHaematologica2010
Rac1 and Rac2 GTPases are necessary for early erythropoietic expansion in the bone marrow but not in the spleen.
Article in Haematologica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 65 citations in OpenAlex.
- The Mechanism of miR-155/miR-15b Axis Contributed to Apoptosis of CD34+ Cells by Upregulation of PD-L1 in Myelodysplastic Syndromes.Mediterranean journal of hematology and infectious diseases · 2023Article
- Metabolic sensor O-GlcNAcylation regulates erythroid differentiation and globin production via BCL11A.Stem cell research & therapy · 2022Article
- Zkscan3 affects erythroblast development by regulating the transcriptional activity of GATA1 and KLF1 in mice.Journal of molecular histology · 2022Article
- The Impact of Different Cell Culture Mediums on CD8+ T Cells Expansion: A Bioinformatics Study.Cell journal · 2022Article
- Sarcomeres Morphology and Z-Line Arrangement Disarray Induced by Ventricular Premature Contractions through the Rac2/Cofilin Pathway.International journal of molecular sciences · 2021Article
- HDAC1 is required for GATA-1 transcription activity, global chromatin occupancy and hematopoiesis.Nucleic acids research · 2021Article
- Pleckstrin-2 is essential for erythropoiesis in β-thalassemic mice, reducing apoptosis and enhancing enucleation.Communications biology · 2021Article
- Differential effects of RASA3 mutations on hematopoiesis are profoundly influenced by genetic background and molecular variant.PLoS genetics · 2020Article
- Cellular dynamics of mammalian red blood cell production in the erythroblastic island niche.Biophysical reviews · 2019Review
- FLT3-ITD cooperates with Rac1 to modulate the sensitivity of leukemic cells to chemotherapeutic agents via regulation of DNA repair pathways.Haematologica · 2019Article
- Mouse Models of Erythropoiesis and Associated Diseases.Methods in molecular biology (Clifton, N.J.) · 2018Review
- Rac1 functions downstream of miR-142 in regulation of erythropoiesis.Haematologica · 2017Article
- Tropomodulin 1 controls erythroblast enucleation via regulation of F-actin in the enucleosome.Blood · 2017Article
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- Identification of a murine erythroblast subpopulation enriched in enucleating events by multi-spectral imaging flow cytometry.Journal of visualized experiments : JoVE · 2014Article
- Rho GTPases in erythroid maturation.Current opinion in hematology · 2014Review
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe small Rho GTPases Rac1 and Rac2 have both overlapping and distinct roles in actin organization, cell survival, and proliferation in various hematopoietic cell lineages. The role of these Rac GTPases in erythropoiesis has not yet been fully elucidated. DESIGN AND
methodsCre-recombinase-induced deletion of Rac1 genomic sequence was accomplished on a Rac2-null genetic background, in mouse hematopoietic cells in vivo. The erythroid progenitors and precursors in the bone marrow and spleen of these genetically engineered animals were evaluated by colony assays and flow cytometry. Apoptosis and proliferation of the different stages of erythroid progenitors and precursors were evaluated by flow cytometry.
resultsErythropoiesis in Rac1(-/-);Rac2(-/-) mice is characterized by abnormal burst-forming unit-erythroid colony morphology and decreased numbers of megakaryocyte-erythrocyte progenitors, erythroid colony-forming units, and erythroblasts in the bone marrow. In contrast, splenic erythropoiesis is increased. Combined Rac1 and Rac2 deficiency compromises proliferation of the megakaryocyte-erythrocyte progenitor population in the bone marrow, while it allows increased survival and proliferation of megakaryocyte-erythrocyte progenitors in the spleen. Conclusions These data suggest that Rac1 and Rac2 GTPases are essential for normal bone marrow erythropoiesis but that they are dispensable for erythropoiesis in the spleen, implying different signaling pathways for homeostatic and stress erythropoiesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.