ArticleAntimicrobial agents and chemotherapy2010
Impact of azithromycin resistance mutations on the virulence and fitness of Chlamydia caviae in guinea pigs.
Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 33 citations in OpenAlex.
- Majority of Treponema pallidum ssp. pallidum MLST allelic profiles in the Czech Republic (2004-2022) belong to two SS14-like clusters.Scientific reports · 2024Article
- Insights into Chlamydia Development and Host Cells Response.Microorganisms · 2024Review
- Direct assessment of possible mutations in the 23S rRNA gene encoding macrolide resistance in Chlamydia trachomatis.PloS one · 2022Article
- An overview of genes and mutations associated with Chlamydiae species' resistance to antibiotics.Annals of clinical microbiology and antimicrobials · 2021Review
- Novel Chlamydia species isolated from snakes are temperature-sensitive and exhibit decreased susceptibility to azithromycin.Scientific reports · 2018Article
- Dynamic energy dependency ofThe Journal of biological chemistry · 2018Article
- Antichlamydial Dimeric Indole Derivatives from Marine Actinomycete Rubrobacter radiotolerans.Planta medica · 2017Article
- Natural Products for the Treatment of Chlamydiaceae Infections.Microorganisms · 2016Review
- Beyond Tryptophan Synthase: Identification of Genes That Contribute to Chlamydia trachomatis Survival during Gamma Interferon-Induced Persistence and Reactivation.Infection and immunity · 2016Article
- Pathogenic Chlamydia Lack a Classical Sacculus but Synthesize a Narrow, Mid-cell Peptidoglycan Ring, Regulated by MreB, for Cell Division.PLoS pathogens · 2016Article
- Chlamydial variants differ in ability to ascend the genital tract in the guinea pig model of chlamydial genital infection.Infection and immunity · 2015Article
- Phosphoproteomic analysis of the Chlamydia caviae elementary body and reticulate body forms.Microbiology (Reading, England) · 2015Article
- Genomic variant representation in a Chlamydia population is dynamic and adaptive with dependence on in vitro and in vivo passage.Pathogens and disease · 2015Article
- Molecular typing of Treponema pallidum in the Czech Republic during 2011 to 2013: increased prevalence of identified genotypes and of isolates with macrolide resistance.Journal of clinical microbiology · 2014Article
- Article
- In vitro susceptibility of urogenital Chlamydia trachomatis strains in a country with high azithromycin consumption rate.Folia microbiologica · 2013Article
- Characterization of the activity and expression of arginine decarboxylase in human and animal Chlamydia pathogens.FEMS microbiology letters · 2012Article
- Resistance to a novel antichlamydial compound is mediated through mutations in Chlamydia trachomatis secY.Antimicrobial agents and chemotherapy · 2012Article
- Impaired fitness and transmission of macrolide-resistant Campylobacter jejuni in its natural host.Antimicrobial agents and chemotherapy · 2012Article
- Effect of inflammatory response on in vivo competition between two chlamydial variants in the guinea pig model of inclusion conjunctivitis.Infection and immunity · 2012Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Azithromycin (AZM) is a major drug used in the treatment and prophylaxis of infections caused by Chlamydia, yet no significant clinical resistance has been reported for these obligate intracellular bacteria. Nevertheless, spontaneous AZM resistance (Azm(r)) arose in vitro at frequencies ranging from 3 x 10(-8) to 8 x 10(-10) for clonal isolates of Chlamydia caviae, which is a natural pathogen of guinea pigs. Sequencing of the unique 23S rRNA gene copy in 44 independent Azm(r) isolates identified single mutations at position A(2058) or A(2059) (Escherichia coli numbering system). While SP(6)AZ(1) (A(2058)C) and SP(6)AZ(2) (A(2059)C) Azm(r) mutants showed growth defects in cell culture and were less pathogenic in the guinea pig ocular infection model than in the parent SP(6), the three isogenic C. caviae isolates grew equally well in the animal. On the other hand, coinoculation of the C. caviae parent strain with one of the Azm(r) strains was detrimental for the mutant strain. This apparent lack of association between pathology and bacterial load in vivo showed that virulence of the two Azm(r) mutants of C. caviae was attenuated. While chlamydial growth in vitro reflects the ability of the bacteria to multiply in permissive cells, survival in the host is a balance between cellular multiplication and clearance by the host immune system. The obligate intracellular nature of Chlamydia may therefore limit emergence of resistance in vivo due to the strength of the immune response induced by the wild-type antibiotic-sensitive bacteria at the time of antibiotic treatment.
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