ArticleMolecular and cellular biology2010
Acetylation of H3 K56 is required for RNA polymerase II transcript elongation through heterochromatin in yeast.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- RNA Polymerase II Dependent Crosstalk between H4K16 Deacetylation and H3K56 Acetylation Promotes Transcription of Constitutively Expressed Genes.Molecular and cellular biology · 2023Article
- Transcription levels of a noncoding RNA orchestrate opposing regulatory and cell fate outcomes in yeast.Cell reports · 2021Article
- The Roles of Chromatin Accessibility in Regulating theJournal of fungi (Basel, Switzerland) · 2021Review
- Regulation of plant architecture by a new histone acetyltransferase targeting gene bodies.Nature plants · 2020Article
- Molecular basis of Tousled-Like Kinase 2 activation.Nature communications · 2018Article
- Article
- Getting down to the core of histone modifications.Chromosoma · 2014Review
- Article
- Uncoupling transcription from covalent histone modification.PLoS genetics · 2014Article
- Acetylated histone H3K56 interacts with Oct4 to promote mouse embryonic stem cell pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Pygo2 regulates histone gene expression and H3 K56 acetylation in human mammary epithelial cells.Cell cycle (Georgetown, Tex.) · 2012Article
- Regulation of white and opaque cell-type formation in Candida albicans by Rtt109 and Hst3.Molecular microbiology · 2011Article
- Autoacetylation of the histone acetyltransferase Rtt109.The Journal of biological chemistry · 2011Article
- A dual role of H4K16 acetylation in the establishment of yeast silent chromatin.The EMBO journal · 2011Article
- Regulation of chromatin assembly/disassembly by Rtt109p, a histone H3 Lys56-specific acetyltransferase, in vivo.The Journal of biological chemistry · 2010Article
- Histone chaperones, histone acetylation, and the fluidity of the chromogenome.Journal of cellular physiology · 2010Article
- Histone H3 serine 57 and lysine 56 interplay in transcription elongation and recovery from S-phase stress.PloS one · 2010Article
- Unveiling novel interactions of histone chaperone Asf1 linked to TREX-2 factors Sus1 and Thp1.Nucleus (Austin, Tex.)Article
- Histone exchange and histone modifications during transcription and aging.Biochimica et biophysica actaReview
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae SIR proteins mediate transcriptional silencing, forming heterochromatin structures at repressed loci. Although recruitment of transcription initiation factors can occur even to promoters packed in heterochromatin, it is unclear whether heterochromatin inhibits RNA polymerase II (RNAPII) transcript elongation. To clarify this issue, we recruited SIR proteins to the coding region of an inducible gene and characterized the effects of the heterochromatic structure on transcription. Surprisingly, RNAPII is fully competent for transcription initiation and elongation at the locus, leading to significant loss of heterochromatin proteins from the region. A search for auxiliary factors required for transcript elongation through the heterochromatic locus revealed that two proteins involved in histone H3 lysine 56 acetylation, Rtt109 and Asf1, are needed for efficient transcript elongation by RNAPII. The efficiency of transcription through heterochromatin is also impaired in a strain carrying the K56R mutation in histone H3. Our results show that H3 K56 modification is required for efficient transcription of heterochromatic locus by RNAPII, and we propose that transcription-coupled incorporation of H3 acetylated K56 (acK56) into chromatin is needed for efficient opening of heterochromatic loci for transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.