ArticleThe American journal of tropical medicine and hygiene2010
Monitoring Leptospira strain collections: the need for quality control.
Article in The American journal of tropical medicine and hygiene, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Navigating cross-reactivity and host species effects in a serological assay: A case study of the microscopic agglutination test for Leptospira serology.PLoS neglected tropical diseases · 2024Article
- Diagnosis of Human Leptospirosis: Comparison of Microscopic Agglutination Test with Recombinant LigA/B Antigen-Based In-House IgM Dot ELISA Dipstick Test and Latex Agglutination Test Using Bayesian Latent Class Model and MAT as Gold Standard.Diagnostics (Basel, Switzerland) · 2022Article
- Spatio-temporal distribution of bovine leptospirosis in Tamil Nadu and a risk factor analysis.Veterinarni medicina · 2021Article
- Molecular Characterization of Leptospira spp. in Environmental Samples from North-Eastern Malaysia Revealed a Pathogenic Strain, Leptospira alstonii.Journal of tropical medicine · 2016Article
- Maintenance of leptospira species in leptospira Vanaporn Wuthiekanun agar.Journal of clinical microbiology · 2014Article
- Interlaboratory agreement of pulsed-field gel electrophoresis identification of Leptospira serovars.The American journal of tropical medicine and hygiene · 2013Article
- Leptospira spp. strain identification by MALDI TOF MS is an equivalent tool to 16S rRNA gene sequencing and multi locus sequence typing (MLST).BMC microbiology · 2012Article
- Molecular detection and speciation of pathogenic Leptospira spp. in blood from patients with culture-negative leptospirosis.BMC infectious diseases · 2011Article
- Comparison of two multilocus sequence based genotyping schemes for Leptospira species.PLoS neglected tropical diseases · 2011Article
- 2010 ACVIM small animal consensus statement on leptospirosis: diagnosis, epidemiology, treatment, and prevention.Journal of veterinary internal medicineArticle
- Variability in results of the microscopic agglutination test in dogs with clinical leptospirosis and dogs vaccinated against leptospirosis.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The purpose of this study was to perform a 16S sequence-based quality control of two Leptospira strain collections. 16S rRNA gene sequencing was used to verify two Leptospira reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify Leptospira beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of Leptospira reference collections.
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