ArticleBMC plant biology2010
Analysis of a c0t-1 library enables the targeted identification of minisatellite and satellite families in Beta vulgaris.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 32 citations in OpenAlex.
- Molecular cytogenetics provides insights into heterochromatin composition and karyotypic evolution in species of the Plebeia group (Apidae: Meliponini).Molecular cytogenetics · 2026Article
- Optimising Exome Captures in Species With Large Genomes Using Species-Specific Repetitive DNA Blocker.Molecular ecology resources · 2025Article
- BeetRepeats: reference sequences for genome and polymorphism annotation in sugar beet and wild relatives.BMC research notes · 2024Article
- The nature and organization of satellite DNAs inFrontiers in plant science · 2023Article
- The Cassandra retrotransposon landscape in sugar beet (Beta vulgaris) and related Amaranthaceae: recombination and re-shuffling lead to a high structural variability.Annals of botany · 2021Article
- Article
- Genome-Wide Analysis of Transposable Elements and Satellite DNAs inFrontiers in plant science · 2020Article
- Repeat Composition of CenH3-chromatin and H3K9me2-marked heterochromatin in Sugar Beet (Beta vulgaris).BMC plant biology · 2016Article
- Next-generation sequencing reveals differentially amplified tandem repeats as a major genome component of Northern Europe's oldest Camellia japonica.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Highly diverse chromoviruses of Beta vulgaris are classified by chromodomains and chromosomal integration.Mobile DNA · 2013Article
- Epigenetic profiling of heterochromatic satellite DNA.Chromosoma · 2011Article
- The first insight into the Taxus genome via fosmid library construction and end sequencing.Molecular genetics and genomics : MGG · 2011Article
- The Ty1-copia families SALIRE and Cotzilla populating the Beta vulgaris genome show remarkable differences in abundance, chromosomal distribution, and age.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundRepetitive DNA is a major fraction of eukaryotic genomes and occurs particularly often in plants. Currently, the sequencing of the sugar beet (Beta vulgaris) genome is under way and knowledge of repetitive DNA sequences is critical for the genome annotation. We generated a c0t-1 library, representing highly to moderately repetitive sequences, for the characterization of the major B. vulgaris repeat families. While highly abundant satellites are well-described, minisatellites are only poorly investigated in plants. Therefore, we focused on the identification and characterization of these tandemly repeated sequences.
resultsAnalysis of 1763 c0t-1 DNA fragments, providing 442 kb sequence data, shows that the satellites pBV and pEV are the most abundant repeat families in the B. vulgaris genome while other previously described repeats show lower copy numbers. We isolated 517 novel repetitive sequences and used this fraction for the identification of minisatellite and novel satellite families. Bioinformatic analysis and Southern hybridization revealed that minisatellites are moderately to highly amplified in B. vulgaris. FISH showed a dispersed localization along most chromosomes clustering in arrays of variable size and number with exclusion and depletion in distinct regions.
conclusionThe c0t-1 library represents major repeat families of the B. vulgaris genome, and analysis of the c0t-1 DNA was proven to be an efficient method for identification of minisatellites. We established, so far, the broadest analysis of minisatellites in plants and observed their chromosomal localization providing a background for the annotation of the sugar beet genome and for the understanding of the evolution of minisatellites in plant genomes.
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