ArticlePloS one2010
HCN channels are not required for mechanotransduction in sensory hair cells of the mouse inner ear.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- A comprehensive review of HCN channel expression and IJournal of neurophysiology · 2025Review
- Article
- HCN channels in the mammalian cochlea: Expression pattern, subcellular location, and age-dependent changes.Journal of neuroscience research · 2021Article
- Uncoordinated maturation of developing and regenerating postnatal mammalian vestibular hair cells.PLoS biology · 2019Article
- Molecular Structure of the Hair Cell Mechanoelectrical Transduction Complex.Cold Spring Harbor perspectives in medicine · 2019Review
- Neural substrates, dynamics and thresholds of galvanic vestibular stimulation in the behaving primate.Nature communications · 2019Article
- TRPV6, TRPM6 and TRPM7 Do Not Contribute to Hair-Cell Mechanotransduction.Frontiers in cellular neuroscience · 2018Article
- Distinct capacity for differentiation to inner ear cell types by progenitor cells of the cochlea and vestibular organs.Development (Cambridge, England) · 2016Article
- Identification and modelling of fast and slow Ih current components in vestibular ganglion neurons.The European journal of neuroscience · 2015Article
- Association of SNPs in LCP1 and CTIF with hearing in 11 year old children: findings from the Avon Longitudinal Study of Parents and Children (ALSPAC) birth cohort and the G-EAR consortium.BMC medical genomics · 2015Article
- The physiology of mechanoelectrical transduction channels in hearing.Physiological reviews · 2014Review
- Mechanotransduction and hyperpolarization-activated currents contribute to spontaneous activity in mouse vestibular ganglion neurons.The Journal of general physiology · 2014Article
- Differences in the expression pattern of HCN isoforms among mammalian tissues: sources and implications.Molecular biology reports · 2014Article
- Uncovering genomic features and maternal origin of korean native chicken by whole genome sequencing.PloS one · 2014Article
- Functional contributions of HCN channels in the primary auditory neurons of the mouse inner ear.The Journal of general physiology · 2013Article
- Hyperpolarization-activated current (I(h)) in vestibular calyx terminals: characterization and role in shaping postsynaptic events.Journal of the Association for Research in Otolaryngology : JARO · 2012Article
- HCN1 and HCN2 proteins are expressed in cochlear hair cells: HCN1 can form a ternary complex with protocadherin 15 CD3 and F-actin-binding filamin A or can interact with HCN2.The Journal of biological chemistry · 2012Article
- HCN channels expressed in the inner ear are necessary for normal balance function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Mechanism of spontaneous activity in afferent neurons of the zebrafish lateral-line organ.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Dendritic HCN channels shape excitatory postsynaptic potentials at the inner hair cell afferent synapse in the mammalian cochlea.Journal of neurophysiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The molecular composition of the hair cell transduction channel has not been identified. Here we explore the novel hypothesis that hair cell transduction channels include HCN subunits. The HCN family of ion channels includes four members, HCN1-4. They were originally identified as the molecular correlates of the hyperpolarization-activated, cyclic nucleotide gated ion channels that carry currents known as If, IQ or Ih. However, based on recent evidence it has been suggested that HCN subunits may also be components of the elusive hair cell transduction channel. To investigate this hypothesis we examined expression of mRNA that encodes HCN1-4 in sensory epithelia of the mouse inner ear, immunolocalization of HCN subunits 1, 2 and 4, uptake of the transduction channel permeable dye, FM1-43 and electrophysiological measurement of mechanotransduction current. Dye uptake and transduction current were assayed in cochlear and vestibular hair cells of wildtype mice exposed to HCN channel blockers or a dominant-negative form of HCN2 that contained a pore mutation and in mutant mice that lacked HCN1, HCN2 or both. We found robust expression of HCNs 1, 2 and 4 but little evidence that localized HCN subunits in hair bundles, the site of mechanotransduction. Although high concentrations of the HCN antagonist, ZD7288, blocked 50-70% of the transduction current, we found no reduction of transduction current in either cochlear or vestibular hair cells of HCN1- or HCN2- deficient mice relative to wild-type mice. Furthermore, mice that lacked both HCN1 and HCN2 also had normal transduction currents. Lastly, we found that mice exposed to the dominant-negative mutant form of HCN2 had normal transduction currents as well. Taken together, the evidence suggests that HCN subunits are not required for mechanotransduction in hair cells of the mouse inner ear.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.