ArticleJournal of bacteriology2010
PepD participates in the mycobacterial stress response mediated through MprAB and SigE.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
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- Systems-level characterization of EGFR kinase inhibitors reveals heterogeneous effects on Mtb-macrophage interactions.bioRxiv : the preprint server for biology · 2025Article
- Two-Component MprAB System Regulates the Expression of Genes Involved in Cell Envelope Biosynthesis inMicroorganisms · 2025Article
- MoaB2, a newly identified transcription factor, binds to σJournal of bacteriology · 2024Article
- Serine protease Rv2569c facilitates transmission of Mycobacterium tuberculosis via disrupting the epithelial barrier by cleaving E-cadherin.PLoS pathogens · 2024Article
- Rel-dependent decrease in the expression of ribosomal protein genes by inhibition of the respiratory electron transport chain inFrontiers in microbiology · 2024Article
- Characteristics of a Temperature-Sensitive Mutant Strain ofVeterinary sciences · 2023Article
- Mycobacterial Regulatory Systems Involved in the Regulation of Gene Expression Under Respiration-Inhibitory Conditions.Journal of microbiology (Seoul, Korea) · 2023Review
- In-depth systems biological evaluation of bovine alveolar macrophages suggests novel insights into molecular mechanisms underlyingFrontiers in microbiology · 2022Article
- Mathematical modelling of SigE regulatory network reveals new insights into bistability of mycobacterial stress response.BMC bioinformatics · 2021Article
- The Epistatic Landscape of Antibiotic Resistance of Different Clades ofAntibiotics (Basel, Switzerland) · 2021Article
- A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- Physiological and Transcriptional Responses ofFrontiers in microbiology · 2020Article
- Understanding high ε-poly-L-lysine production by Streptomyces albulus using pH shock strategy in the level of transcriptomics.Journal of industrial microbiology & biotechnology · 2019Article
- Multiple transcription factors co-regulate the Mycobacterium tuberculosis adaptation response to vitamin C.BMC genomics · 2019Article
- Mycobacterium smegmatis HtrA Blocks the Toxic Activity of a Putative Cell Wall Amidase.Cell reports · 2019Article
- The crystal structure of Mycobacterium tuberculosis high-temperature requirement A protein reveals an autoregulatory mechanism.Acta crystallographica. Section F, Structural biology communications · 2018Article
- SyNDI: synchronous network data integration framework.BMC bioinformatics · 2018Article
- A temporal proteome dynamics study reveals the molecular basis of induced phenotypic resistance in Mycobacterium smegmatis at sub-lethal rifampicin concentrations.Scientific reports · 2017Article
- Release of an HtrA-Like Protease from the Cell Surface of ThermophilicFrontiers in microbiology · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Currently, one-third of the world's population is believed to be latently infected with Mycobacterium tuberculosis. The mechanisms by which M. tuberculosis establishes latent infection remain largely undefined. mprAB encodes a two-component signal transduction system required by M. tuberculosis for aspects of persistent infection. MprAB regulates a large and diverse group of genetic determinants in response to membrane stress, including the extracytoplasmic function (ECF) sigma factor sigE and the HtrA-like serine protease pepD. Recent studies have demonstrated that PepD functions as both a protease and chaperone in vitro. In addition, inactivation of pepD alters the virulence of M. tuberculosis in a mouse model system of infection. Here, we demonstrate that PepD plays an important role in the stress response network of Mycobacterium mediated through MprAB and SigE. In particular, we demonstrate that the protease activity of PepD requires the PDZ domain, in addition to the catalytic serine at position 317. pepD expression initiates from at least three promoters in M. tuberculosis, including one that is regulated by SigE and is located upstream of the mprA coding sequence. Deletion of pepD or mprAB in Mycobacterium smegmatis and M. tuberculosis alters the stress response phenotypes of these strains, including increasing sensitivity to SDS and cell wall antibiotics and upregulating the expression of stress-responsive determinants, including sigE. Taking these data together, we hypothesize that PepD utilizes its PDZ domain to recognize and process misfolded proteins at the cell membrane, leading to activation of the MprAB and SigE signaling pathways and subsequent establishment of a positive feedback loop that facilitates bacterial adaptation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.