ArticleApplied and environmental microbiology2010
Performance assessment PCR-based assays targeting bacteroidales genetic markers of bovine fecal pollution.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- QMRA for assessing treatment needs of surface water for drinking: trends and challenges in fecal pathogen quantification.Journal of water and health · 2025Review
- Distribution of host-specific Bacteriodales marker genes in water sources of selected rural areas of Vhembe District, South Africa.Scientific reports · 2024Article
- The Tomato Brown Rugose Fruit Virus Movement Protein Gene Is a Novel Microbial Source Tracking Marker.Applied and environmental microbiology · 2023Article
- Tomato brown rugose fruit virus Mo gene is a novel microbial source tracking marker.bioRxiv : the preprint server for biology · 2023Article
- Genetic fecal source identification in urban streams impacted by municipal separate storm sewer system discharges.PloS one · 2023Article
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Microbial Source Tracking as a Method of Determination of Beach Sand Contamination.International journal of environmental research and public health · 2022Article
- Microbial Source Tracking of Fecal Indicating Bacteria in Coral Reef Waters, Recreational Waters, and Groundwater of Saipan by Real-Time Quantitative PCR.Frontiers in microbiology · 2020Article
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- Evidence of Genetic Fecal Marker Interactions between Water Column and Periphyton in Artificial Streams.ACS omega · 2018Article
- PCR data and comparative performance ofData in brief · 2018Article
- Toward Forensic Uses of Microbial Source Tracking.Microbiology spectrum · 2018Article
- Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities.Frontiers in microbiology · 2015Review
- Persistence of Bacteroides ovatus under simulated sunlight irradiation.BMC microbiology · 2014Article
- Biotic interactions and sunlight affect persistence of fecal indicator bacteria and microbial source tracking genetic markers in the upper Mississippi river.Applied and environmental microbiology · 2014Article
- Age-related shifts in the density and distribution of genetic marker water quality indicators in cow and calf feces.Applied and environmental microbiology · 2014Article
- Performance characteristics of qPCR assays targeting human- and ruminant-associated bacteroidetes for microbial source tracking across sixteen countries on six continents.Environmental science & technology · 2013Article
- Evaluation of bovine feces-associated microbial source tracking markers and their correlations with fecal indicators and zoonotic pathogens in a Brisbane, Australia, reservoir.Applied and environmental microbiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are numerous PCR-based assays available to characterize bovine fecal pollution in ambient waters. The determination of which approaches are most suitable for field applications can be difficult because each assay targets a different gene, in many cases from different microorganisms, leading to variation in assay performance. We describe a performance evaluation of seven end-point PCR and real-time quantitative PCR (qPCR) assays reported to be associated with either ruminant or bovine feces. Each assay was tested against a reference collection of DNA extracts from 247 individual bovine fecal samples representing 11 different populations and 175 fecal DNA extracts from 24 different animal species. Bovine-associated genetic markers were broadly distributed among individual bovine samples ranging from 39 to 93%. Specificity levels of the assays spanned 47.4% to 100%. End-point PCR sensitivity also varied between assays and among different bovine populations. For qPCR assays, the abundance of each host-associated genetic marker was measured within each bovine population and compared to results of a qPCR assay targeting 16S rRNA gene sequences from Bacteroidales. Experiments indicate large discrepancies in the performance of bovine-associated assays across different bovine populations. Variability in assay performance between host populations suggests that the use of bovine microbial source-tracking applications will require a priori characterization at each watershed of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.