ArticleApplied and environmental microbiology2010
Tool for quantification of staphylococcal enterotoxin gene expression in cheese.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 93 citations in OpenAlex.
- Expression of Panton-Valentine leukocidin mRNA among Staphylococcus aureus isolates associates with specific clinical presentations.PloS one · 2013Trial
- Review
- The Effects of Low Concentrations of Nisin on Biofilm Formation byPathogens (Basel, Switzerland) · 2025Article
- Co-Occurrence ofToxins · 2022Article
- Evaluation of suitable reference genes for normalization of quantitative reverse transcription PCR analyses in Clavibacter michiganensis.MicrobiologyOpen · 2019Article
- Contribution of natural milk culture to microbiota, safety and hygiene of raw milk cheese produced in alpineItalian journal of food safety · 2018Article
- Article
- Comparative Transcriptomics Reveals Discrete Survival Responses ofFrontiers in microbiology · 2017Article
- Transcriptomic and metabolic responses of Staphylococcus aureus in mixed culture with Lactobacillus plantarum, Streptococcus thermophilus and Enterococcus durans in milk.Journal of industrial microbiology & biotechnology · 2016Article
- Selection of suitable reference genes for gene expression studies in Staphylococcus capitis during growth under erythromycin stress.Molecular genetics and genomics : MGG · 2016Article
- Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk.Toxins · 2016Article
- Identification and Characterization of a Novel Staphylococcal Emetic Toxin.Applied and environmental microbiology · 2015Article
- Article
- Following pathogen development and gene expression in a food ecosystem: the case of a Staphylococcus aureus isolate in cheese.Applied and environmental microbiology · 2014Article
- Quantification of yeast and bacterial gene transcripts in retail cheeses by reverse transcription-quantitative PCR.Applied and environmental microbiology · 2013Article
- Growth of aerobic ripening bacteria at the cheese surface is limited by the availability of iron.Applied and environmental microbiology · 2012Article
- Article
- Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cheese is a complex and dynamic microbial ecosystem characterized by the presence of a large variety of bacteria, yeasts, and molds. Some microorganisms, including species of lactobacilli or lactococci, are known to contribute to the organoleptic quality of cheeses, whereas the presence of other microorganisms may lead to spoilage or constitute a health risk. Staphylococcus aureus is recognized worldwide as an important food-borne pathogen, owing to the production of enterotoxins in food matrices. In order to study enterotoxin gene expression during cheese manufacture, we developed an efficient procedure to recover total RNA from cheese and applied a robust strategy to study gene expression by reverse transcription-quantitative PCR (RT-qPCR). This method yielded pure preparations of undegraded RNA suitable for RT-qPCR. To normalize RT-qPCR data, expression of 10 potential reference genes was investigated during S. aureus growth in milk and in cheese. The three most stably expressed reference genes during cheese manufacture were ftsZ, pta, and gyrB, and these were used as internal controls for RT-qPCR of the genes sea and sed, encoding staphylococcal enterotoxins A and D, respectively. Expression of these staphylococcal enterotoxin genes was monitored during the first 72 h of the cheese-making process, and mRNA data were correlated with enterotoxin production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.