ArticleApplied and environmental microbiology2010
Viral multiplex quantitative PCR assays for tracking sources of fecal contamination.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- Resilient performance indicator virus for membrane filtration considering surface charge, hydrophobicity, and morphology.Applied and environmental microbiology · 2026Article
- Characterizing fecal bioaerosols in dairy barns using microbial source tracking markers and bovine enteric viruses.Microbiology spectrum · 2026Article
- Comparison of two sentinel speciesMicrobiology spectrum · 2026Article
- Optimization of a Semi-nested PCR Protocol for Amplifying the Entire Spike Protein Region to Identify SARS-CoV-2 Variants in Wastewater.Food and environmental virology · 2026Article
- Optimized protocol for direct extraction of SARS-CoV-2 RNA from raw wastewater samples (ANRS 0160).MethodsX · 2025Article
- PMAxx-RT-qPCR to Determine Human Norovirus Inactivation Following High-Pressure Processing of Oysters.Food and environmental virology · 2024Article
- Improved performance of nucleic acid-based assays for genetically diverse norovirus surveillance.Applied and environmental microbiology · 2023Article
- A Comprehensive Review for the Surveillance of Human Pathogenic Microorganisms in Shellfish.Microorganisms · 2023Review
- Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration.Food and environmental virology · 2023Article
- Article
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- First Isolation and Identification of Homologous Recombination Events of Porcine Adenovirus from Wild Boar.Viruses · 2022Article
- High Rates of Detection and Molecular Characterization of Porcine Adenovirus Serotype 5 (Pathogens (Basel, Switzerland) · 2022Article
- Escherichia coli, Species C Human Adenovirus, and Enterovirus in Water Samples Consumed in Rural Areas of Goiás, Brazil.Food and environmental virology · 2022Article
- Sewage Promotes Vibrio vulnificus Growth and Alters Gene Transcription in Vibrio vulnificus CMCP6.Microbiology spectrum · 2022Article
- Removal of viruses from their cocktail solution by liquid-crystalline water-treatment membranes.Polymer journal · 2022Article
- Elimination of SARS-CoV-2 along wastewater and sludge treatment processes.Water research · 2021Article
- Physicochemical, microbiological quality, and risk assessment of water consumed by a quilombola community in midwestern Brazil.Environmental science and pollution research international · 2021Article
- Low circulation of Influenza A and coinfection with SARS-CoV-2 among other respiratory viruses during the COVID-19 pandemic in a region of southern Brazil.Journal of medical virology · 2021Article
- Epidemiological surveillance of SARS-CoV-2 by genome quantification in wastewater applied to a city in the northeast of France: Comparison of ultrafiltration- and protein precipitation-based methods.International journal of hygiene and environmental health · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human and animal fecal pollution of the environment presents a risk to human health because of the presence of pathogenic viruses and bacteria. To distinguish between human and animal sources of pollution, we designed specific real-time reverse transcription (RT)-PCR assays for human and animal enteric viruses, including norovirus genogroups I, II, and III; porcine adenovirus types 3 and 5; ovine adenovirus; atadenovirus; and human adenovirus species C and F, which are excreted by infected humans, pigs, cattle, sheep, deer, and goats, and for the detection of F+ RNA bacteriophage genogroups I to IV, which are associated with human and animal wastes. The sensitivity of this viral toolbox (VTB) was tested against 10-fold dilution series of DNA plasmids that carry the target sequences of the respective viruses and was shown to detect at least 10 plasmid copies for each assay. A panel of human and animal enteric and respiratory viruses showed these assays to be highly sensitive and specific to their respective targets. The VTB was used to detect viruses in fecal and environmental samples, including raw sewage and biosolids from municipal sewage treatment plants, abattoir sewage, and fecally contaminated shellfish and river water, which were likely to contain animal or human viruses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.