ArticleFungal genetics and biology : FG & B2010
Deletion of Candida albicans SPT6 is not lethal but results in defective hyphal growth.
Article in Fungal genetics and biology : FG & B, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Insights into Spt6: a histone chaperone that functions in transcription, DNA replication, and genome stability.Trends in genetics : TIG · 2023Review
- Transcriptional control of hyphal morphogenesis in Candida albicans.FEMS yeast research · 2020Review
- Negative regulation of filamentous growth in Candida albicans by Dig1p.Molecular microbiology · 2017Article
- The Candida albicans Histone Acetyltransferase Hat1 Regulates Stress Resistance and Virulence via Distinct Chromatin Assembly Pathways.PLoS pathogens · 2015Article
- The role of Candida albicans SPT20 in filamentation, biofilm formation and pathogenesis.PloS one · 2014Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- The cellular protein SPT6 is required for efficient replication of human cytomegalovirus.Journal of virology · 2012Article
- Spt6 is required for heterochromatic silencing in the fission yeast Schizosaccharomyces pombe.Molecular and cellular biology · 2011Article
- Control of chromatin structure by spt6: different consequences in coding and regulatory regions.Molecular and cellular biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
As a means to study surface proteins involved in the yeast to hypha transition, human monoclonal antibody fragments (single-chain variable fragments, scFv) have been generated that bind to antigens expressed on the surface of Candida albicans yeast and/or hyphae. A cDNA expression library was constructed from hyphae, and screened for immunoreactivity with scFv5 as a means to identify its cognate antigen. A reactive clone contained the 3' end of the C. albicans gene, orf 19.7136, designated SPT6 based on homology to Saccharomyces cerevisiae, where its product functions as a transcription elongation factor. A mutant containing a homozygous deletion of SPT6 was isolated, demonstrating that unlike S. cerevisiae, deletion of this gene in C. albicans is not lethal. Growth of this strain was severely impaired, however, as was its capacity to undergo filamentous growth. Reactivity with scFv5 was not detected in the mutant strain, although its impaired growth complicates the interpretation of this finding. To assess C. albicansSPT6 function, expression of the C. albicans gene was induced in a defined S. cerevisiaespt6 mutant. Partial complementation was seen, confirming that the C. albicans and S. cerevisiae genes are functionally related in these species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.